Cyanine 3, SE
Chemical Name: 2-[3-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity: ≥95%
Biological Activity
Cyanine 3, SE is an orange fluorescent dye for labeling of amines. Emission maximum ~564 nm.Optical Data for Cyanine 3, SE
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 550 nm |
| λem | 570 nm |
| Extinction Coefficient (ε) | 150000 M-1cm-1 |
| Quantum Yield (φ) | 0.31 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.04 |
| Correction Factor 280 | 0.09 |
| Application | Flow Cytometry |
| Alternative For | Alexa Fluor 546; DyLight 549 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters.
Shin et al.
Nucleic Acids Res., 2014;42:e90
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