Cyanine 3 Tyramide
Chemical Name: 2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Purity: ≥95%
Biological Activity
Cyanine 3 Tyramide is an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.Optical Data for Cyanine 3 Tyramide
| Emission Color | Orange |
|---|---|
| λabs | 550 nm |
| λem | 563 nm |
| Extinction Coefficient (ε) | 150000 M-1cm-1 |
| Quantum Yield (φ) | 0.31 |
| Closest Laser line | 532 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size.
Schriml et al.
Biotechniques, 1999;27:608 -
Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification.
Hopman et al.
J.Histochem.Cytochem., 1998;46:771 -
Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization.
van Gijlswijk et al.
J.Histochem.Cytochem., 1997;45:375 -
Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide.
Schmidt et al.
J.Histochem.Cytochem., 1997;45:365 -
Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification.
Zhao et al.
PLoS One, 2013;8:e73750
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