Cyanine 5, SE
Chemical Name: 2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity: ≥95%
Biological Activity
Key information: Cyanine 5, SE is a widely used red fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.Application: Suitable for fluorescence microscopy, confocal microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 649 nm and 666 nm, respectively; quantum yield = 0.2; extinction coefficient = 250,000 M-1cm-1; A280 correction factor = 0.04.
For more information on how Cyanine 5, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Optical Data for Cyanine 5, SE
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 649 nm |
| λem | 666 nm |
| Extinction Coefficient (ε) | 250000 M-1cm-1 |
| Quantum Yield (φ) | 0.2 |
| Closest Laser line | 647 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.03 |
| Correction Factor 280 | 0.04 |
| Application | Fluorescence Microscopy, Confocal Microscopy, Flow cytometry |
| Alternative For | Alexa Fluor 647; DyLight 649 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters.
Shin et al.
Nucleic Acids Res., 2014;42:e90
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Citation for Cyanine 5, SE
The citations listed below are publications that use Tocris products. Selected citations for Cyanine 5, SE include:
1 Citation: Showing 1 - 1
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Directed Evolution of Fluorescent Proteins in Bacteria.
Authors: Erik A Et al.
Methods Mol Biol 2022;2564:75-97
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