Cyanine 5 Tyramide
Chemical Name: 2-[5-[1,3-Dihydro-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino]-6-oxohexyl]3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Purity: ≥95%
Biological Activity
Cyanine 5 Tyramide is a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.Optical Data for Cyanine 5 Tyramide
| Emission Color | Red |
|---|---|
| λabs | 651 nm |
| λem | 665 nm |
| Extinction Coefficient (ε) | 250000 M-1cm-1 |
| Closest Laser line | 647 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer.
Chanda et al.
PLoS One, 2017;12:e0177639 -
Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer.
Matsumoto et al.
Br.J.Cancer, 2017;117:1537 -
Identification of differentially expressed genes from ovarian cancer cells by MICROMAX cDNA microarray system.
Wong et al.
Biotechniques, 2001;30:670
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