Digoxigenin Alexa Fluor® 488-conjugated Antibody

Catalog #: IC7520G
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IC7520G

Digoxigenin-labeled ATF6 Antibody in A549 Human Cell Line.

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Digoxigenin Alexa Fluor® 488-conjugated Antibody Summary

Specificity

Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.

Source

Monoclonal Mouse IgG_2A Clone # 611621

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLH-coupled Digoxigenin

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

1:10 dilution

See below

Immunocytochemistry

1:10 dilution

Immersion fixed A549 human lung carcinoma cell line using digoxigenin-labeled Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (Catalog # MAB71527) conjugated to digoxigenin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Digoxigenin-labeled ATF6 Antibody in A549 Human Cell Line. ATF6 was detected in immersion fixed A549 human lung carcinoma cell line using digoxigenin-labeled Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (MAB71527) at 8 µg/mL for 3 hours at room temperature. Cells were stained using Anti-Mouse Anti-Digoxigenin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC7520G) at 1:10 dilution and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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Digoxigenin-labeled Netrin-4 Antibody in Mouse Brain. Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using digoxigenin-conjugated Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Digoxigenin Alexa Fluor® 488-conjugated Monoclonal Antibody (green; Catalog # IC7520G) at a final concentration of 1X (1:10 dilution) and counterstained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References

Décarie, A.A. et al. (1994) Peptides 15(3):511.
Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Alternate Names

DIG; Digoxigenin

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Digoxigenin Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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