Digoxigenin Antibody

Digoxigenin in Human Liver. Digoxigenin was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Digoxigenin Monoclonal Antibody (Catalog # MAB10386) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Digoxigenin labeled anti-Vimentin antibodies bound in the bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Digoxigenin in HeLa Human Cell Line. Digoxigenin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Digoxigenin Monoclonal Antibody (Catalog # MAB10386) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm incubated with Digoxigenin-labeled anti-Vimentin antibody. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.