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Digoxigenin Antibody

Catalog #: MAB7520
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7520
MAB7520-SP
Digoxigenin in Mouse Brain.
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Citations (4)
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Digoxigenin Antibody Summary

Specificity
Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
Source
Monoclonal Mouse IgG2A Clone # 611621
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH-coupled Digoxigenin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Digoxigenin antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

Digoxigenin in Mouse Brain. Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin at 15 µg/mL overnight at 4 °C. Tissue was stained using 25 µg/mL Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB7520) followed by incubation with NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007). Tissue was counter-stained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References
  1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
  2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
  3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.
Alternate Names
DIG; Digoxigenin

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Citations for Digoxigenin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. An antibody-based molecular switch for continuous small-molecule biosensing
    Authors: Thompson, IAP;Saunders, J;Zheng, L;Hariri, AA;Maganzini, N;Cartwright, AP;Pan, J;Yee, S;Dory, C;Eisenstein, M;Vuckovic, J;Soh, HT;
    Science advances
    Species: N/A
    Sample Types: Complex Sample Type
    Applications: Bioassay
  2. Stress-induced plasticity of a CRH/GABA projection disrupts reward behaviors in mice
    Authors: MT Birnie, AK Short, GB de Carvalh, L Taniguchi, BG Gunn, AL Pham, CA Itoga, X Xu, LY Chen, SV Mahler, Y Chen, TZ Baram
    Nature Communications, 2023-02-25;14(1):1088.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Tau-induced nuclear envelope invagination causes a toxic accumulation of mRNA in Drosophila
    Authors: GL Cornelison, SA Levy, T Jenson, B Frost
    Aging Cell, 2018-11-09;0(0):e12847.
    Species: Drosophila
    Sample Types: Whole Tissue
    Applications: IHC
  4. DDX17 is an essential mediator of sterile NLRC4 inflammasome activation by retrotransposon RNAs
    Authors: Shao-bin Wang, Siddharth Narendran, Shuichiro Hirahara, Akhil Varshney, Felipe Pereira, Ivana Apicella et al.
    Science Immunology

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