Digoxigenin Biotinylated Antibody

Catalog #: BAM7520 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAM7520

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

1 Image

All Digoxigenin Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Digoxigenin Biotinylated Antibody Summary

Specificity

Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.

Source

Monoclonal Mouse IgG_2A Clone # 611621

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLH-coupled Digoxigenin

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Customers also Viewed

Mouse ICOS Alexa Fluor® 488-conjugated Antibody

Cat # FAB168G

Ghrelin antibody in Mouse Stomach by Immunohistochemistry (IHC-Fr).

Human/Mouse Ghrelin Antibody

Cat # MAB8200

Citations (11)

Human ERK1 Antibody

Cat # MAB1940

Citations (1)

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Digoxigenin in Mouse Brain. Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin at 15 µg/mL overnight at 4 °C. Tissue was stained using the Digoxigenin Biotinylated Monoclonal Antibody (brown; Catalog # BAM7520) at 3 µg/mL followed by Streptavidin-HRP incubation. Specific staining was localized to vasculature. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References