Directed In Vivo Angiogenesis Assay
Directed In Vivo Angiogenesis Assay Summary
The Directed In Vivo Angiogenesis Assay (DIVAA) Activation Kit was designed for assessing angiogenesis activation.Why Use Directed In Vivo Angiogenesis Assay?
DIVAA is the first in vivo system for the study of angiogenesis that provides quantitative and reproducible results. During the course of the assay, implant grade silicone cylinders closed at one end, called angioreactors, are filled with 20 μl of Cultrex Basement Membrane Extract (BME) premixed with or without angiogenic-modulating factors. These angioreactors are then implanted subcutaneously in the dorsal flank of nude mice. Accompanied with the onset of angiogenesis, vascular endothelial cells proceed to grow into the BME and form vessels in the angioreactor. As early as nine days post-implantation, there are enough cells to determine an effective dose response to angiogenic modulating factors. The sleek design of the angioreactor provides a standardized platform for reproducible and quantifiable in vivo angiogenesis assays. Compared to the plug assay, the angioreactor prevents assay errors due to absorption of the basement membrane extract by the mouse. In addition, the angioreactor uses only a fraction of the materials conserving both BME and test compounds used, and up to four angioreactors may be implanted in each mouse, allowing for greater statistical power. DIVAA has been used in a variety of investigations.
Kit Contents
• DIVAA BME
• DIVAA FGF-2 Positive Control
• DIVAA Cell Sperse
• DIVAA FGF-2 (300 ng)/VEGF(100 ng)
• DIVAA Angioreactor
• DIVAA Wash Buffer
• DIVAA FITC-Lectin 200X
• 25X FITC-Lectin Diluent
• Heparin Solution
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for Directed In Vivo Angiogenesis Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Lysosome purinergic receptor P2X4 regulates neoangiogenesis induced by microvesicles from sarcoma patients
Authors: W Palinski, M Monti, R Camerlingo, I Iacobucci, S Bocella, F Pinto, C Iannuzzi, G Mansueto, S Pignatiell, F Fazioli, M Gallo, L Marra, F Cozzolino, A De Chiara, P Pucci, A Bilancio, F de Nigris
Cell Death & Disease, 2021-08-17;12(9):797. 2021-08-17
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High Aldehyde Dehydrogenase Activity Identifies a Subset of Human Mesenchymal Stromal Cells with Vascular Regenerative Potential.
Authors: Sherman S, Kuljanin M, Cooper T, Putman D, Lajoie G, Hess D
Stem Cells, 2017-04-03;35(6):1542-1553. 2017-04-03
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Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T
J Biol Chem, 2015-07-24;290(36):22212-24. 2015-07-24
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Syndecan-4 is a major syndecan in primary human endothelial cells in vitro, modulated by inflammatory stimuli and involved in wound healing.
Authors: Vuong T, Reine T, Sudworth A, Jenssen T, Kolset S
J Histochem Cytochem, 2015-01-09;63(4):280-92. 2015-01-09
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Soluble carcinoembryonic antigen activates endothelial cells and tumor angiogenesis.
Authors: Bramswig K, Poettler M, Unseld M, Wrba F, Uhrin P, Zimmermann W, Zielinski C, Prager G
Cancer Res, 2013-10-11;73(22):6584-96. 2013-10-11
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Cryptosporidium parvum induces an endoplasmic stress response in the intestinal adenocarcinoma HCT-8 cell line.
Authors: Morada M, Pendyala L, Wu G, Merali S, Yarlett N
J Biol Chem, 2013-08-28;288(42):30356-64. 2013-08-28
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A urokinase receptor-derived peptide inhibiting VEGF-dependent directional migration and vascular sprouting.
Authors: Bifulco K, Longanesi-Cattani I, Liguori E, Arra C, Rea D, Masucci M, De Rosa M, Pavone V, Stoppelli M, Carriero M
Mol Cancer Ther, 2013-08-12;12(10):1981-93. 2013-08-12
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Liver X receptor activation reduces angiogenesis by impairing lipid raft localization and signaling of vascular endothelial growth factor receptor-2.
Authors: Noghero A, Perino A, Seano G, Saglio E, Lo Sasso G, Veglio F, Primo L, Hirsch E, Bussolino F, Morello F
Arterioscler Thromb Vasc Biol, 2012-06-21;32(9):2280-8. 2012-06-21
FAQs
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Around the angioreactor in the mouse, a capsule forms with time. When harvesting the angioreactor, it is difficult to dissect the capsule tissue from the opening of the angioreactor. There is a plug of tissue growing into the chamber. Is this normal? Do you have any recommendations on how to handle chambers like this?
Growth of connective tissue into the angioreactor during angiogenesis is normal. The amount of connective tissue is dependent on the strain of mice, treatment, and response. The cell dissociation step may be increased up to 3 hours to improve dissociation/degradation, and any remaining connective tissue may be removed. If this does not solve the problem, it may be more appropriate to use the Optional Protocol for Dextran-FITC Detection (which measures capillary volume and discards cellular/connective tissue debris).
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