DMHBO+
Chemical Name: 4-(4-((Z)-4-Hydroxy-3,5-dimethoxybenzylidene)-2-((hydroxyimino)methyl)-5-oxo-4,5-dihydro-1H-imidazol-1-yl)-N,N,N-trimethylbenzenaminium iodide
Purity: ≥98%
Biological Activity
DMHBO+ is a cationic fluorophore. Its fluorescence is activated by binding to chili aptamer (Kd = 12 nM). Chili-DMHBO+ complex mimics red fluorescent proteins, suitable for imaging RNA in cells. It is an ideal FRET donor to the rhodamine dye Atto 590 and has applications in FRET-based analytical RNA systems. Excitation/emission maxima (λ) = 456/592 nm; quantum yield = 0.1; stokes shift = 136 nm.Optical Data for DMHBO+
| λabs | 456 nm |
|---|---|
| λem | 592 nm |
| Quantum Yield (φ) | 0.1 |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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