Mouse IgG NorthernLights™ NL493-conjugated Antibody
Mouse IgG NorthernLights™ NL493-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection Mouse IgG Primary Antibody by Immunocytochemistry. Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).
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Preparation and Storage
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Mouse IgG NorthernLights™ NL493-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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ACE-2 Blockade & TMPRSS2 Inhibition Mitigate SARS-CoV-2 Severity Following Cigarette Smoke Exposure in Airway Epithelial Cells In Vitro
Authors: Hussain, SS;Libby, EF;Lever, JEP;Tipper, JL;Phillips, SE;Mazur, M;Li, Q;Campos-Gómez, J;Harrod, KS;Rowe, SM;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification
Authors: GAE Pickering, F Felix-Ilem, MJ Clark, K Kocsy, J Simpson, I Bellantuon, A Gartland, JM Wilkinson, K Hatzikotou, E Kiss-Toth
International Journal of Molecular Sciences, 2022-08-16;23(16):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of iPSC line (GLNNFi001-A) from peripheral blood mononuclear cells of a patient with macular corneal dystrophy
Authors: K Chakrabart, KN Prashanthi, R Shetty, S Argulwar, N Jeyabalan, A Ghosh
Stem Cell Research, 2022-04-22;62(0):102789.
Species: Human
Sample Types: Transduced Whole Cells
Applications: ICC -
Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment
Authors: Ameneh Mehri Ghahfarrokhi, Mohammad Saeid Jami, Morteza Hashemzadeh Chaleshtori
Int J Mol Cell Med
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The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer
Authors: G Kaufman, NM Kiburi, D Skrtic
Biosci. Rep., 2020-06-26;40(6):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice
Authors: C Tashita, M Hoshi, A Hirata, K Nakamoto, T Ando, T Hattori, Y Yamamoto, H Tezuka, H Tomita, A Hara, K Saito
World J. Gastroenterol., 2020-03-07;26(9):918-932.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Optimized Modeled Myofascial Release Enhances Wound Healing in 3-Dimensional Bioengineered Tendons: Key Roles for Fibroblast Proliferation and Collagen Remodeling
Authors: M Zein-Hammo, PR Standley
J Manipulative Physiol Ther, 2019-11-23;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Enhancing the Three-Dimensional Structure of Adherent Gingival Fibroblasts and Spheroids via a Fibrous Protein-Based Hydrogel Cover
Cells Tissues Organs (Print), 2016-08-30;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Amantadine Alleviates Postoperative Cognitive Dysfunction Possibly by Increasing Glial Cell Line-derived Neurotrophic Factor in Rats
Authors: Junfeng Zhang, Hongying Tan, Wei Jiang, Zhiyi Zuo
Anesthesiology
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Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat.
Authors: Wang Z, Huang W, Zuo Z
J Neuroinflammation, 2014-03-25;11(0):56.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Isoflurane Induces Learning Impairment That Is Mediated by Interleukin 1 beta in Rodents
Authors: Lin Cao, Liaoliao Li, Daowei Lin, Zhiyi Zuo
PLoS ONE
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Inhibition of exocytosis or endocytosis blocks activity-dependent redistribution of synapsin.
Authors: Orenbuch A, Ayelet O, Shulman Y, Yoav S, Lipstein N, Noa L, Bechar A, Amit B, Lavy Y, Yotam L, Brumer E, Eliaz B, Vasileva M, Mariya V, Kahn J, Joy K, Barki-Harrington L, Liza BH, Kuner T, Thomas K, Gitler D, Daniel G
J. Neurochem., 2011-12-02;120(2):248-58.
Applications: ICC -
A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells.
Authors: Gopalakrishna-Pillai S, Iverson LE
PLoS ONE, 2011-06-15;6(6):e20663.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
NorthernLights in slide-based cytometry and microscopy.
Authors: Wessels JT, Busse AC, Mahrt J, Hoffschulte B, Mueller GA, Tarnok A, Mittag A
Cytometry A, 2010-05-01;77(5):420-8.
Species: Human
Sample Types: Whole Cells
Applications: IHC
FAQs
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Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
Reviews for Mouse IgG NorthernLights™ NL493-conjugated Antibody
Average Rating: 5 (Based on 3 Reviews)
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Works great as a secondary antibody on western blots and Immunofluorescences. We used at 1:100 for western on many different mouse antibodies.
We used this on a cell culture in which cells were adhered to slides and stained for individual proteins. This secondary was used 1:100 for 1 h at room temperature, and worked perfectly. Was able to read on our fluorescence microscope.
