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Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Catalog #: NL004
40 Citations 1 Review Datasheet / COA / SDS
NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
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NL004
Detection of Rabbit IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (40)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rabbit IgG NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Rabbit IgG Primary Antibody by Immunocytochemistry. Detection of human Ki-67 by incubating cells with 3 μg/mL of Rabbit anti-Human Ki-67 monoclonal antibody (MAB7617) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rabbit IgG NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. ACE-2 Blockade & TMPRSS2 Inhibition Mitigate SARS-CoV-2 Severity Following Cigarette Smoke Exposure in Airway Epithelial Cells In Vitro
    Authors: Hussain, SS;Libby, EF;Lever, JEP;Tipper, JL;Phillips, SE;Mazur, M;Li, Q;Campos-Gómez, J;Harrod, KS;Rowe, SM;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Microglial P2X4 receptors promote ApoE degradation and contribute to memory deficits in Alzheimer’s disease
    Authors: Jennifer Hua, Elvira Garcia de Paco, Nathalie Linck, Tangui Maurice, Catherine Desrumaux, Bénédicte Manoury et al.
    Cellular and Molecular Life Sciences
  3. Studies on a widely-recognized snail model species (Lymnaea stagnalis) provide further evidence that vertebrate steroids do not have a hormonal role in the reproduction of mollusks
    Authors: István Fodor, Tamar Schwarz, Bence Kiss, Antal Tapodi, János Schmidt, Alex R. O. Cousins et al.
    Front Endocrinol (Lausanne)
  4. The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification
    Authors: GAE Pickering, F Felix-Ilem, MJ Clark, K Kocsy, J Simpson, I Bellantuon, A Gartland, JM Wilkinson, K Hatzikotou, E Kiss-Toth
    International Journal of Molecular Sciences, 2022-08-16;23(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Generation of iPSC line (GLNNFi001-A) from peripheral blood mononuclear cells of a patient with macular corneal dystrophy
    Authors: K Chakrabart, KN Prashanthi, R Shetty, S Argulwar, N Jeyabalan, A Ghosh
    Stem Cell Research, 2022-04-22;62(0):102789.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: ICC
  6. Critical role of FPR1 in splenocyte migration into brain to worsen inflammation and ischemic brain injury in mice
    Authors: J Li, MD Chordia, Y Zhang, H Zong, D Pan, Z Zuo
    Theranostics, 2022-03-21;12(7):3024-3044.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Metabolic reprogramming mediates hippocampal microglial M1 polarization in response to surgical trauma causing perioperative neurocognitive disorders
    Authors: G Luo, X Wang, Y Cui, Y Cao, Z Zhao, J Zhang
    Journal of Neuroinflammation, 2021-11-13;18(1):267.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice
    Authors: D Halperin, A Stavsky, R Kadir, M Drabkin, O Wormser, Y Yogev, V Dolgin, R Proskorovs, Y Perez, H Nudelman, O Stoler, B Rotblat, T Lifschytz, A Lotan, G Meiri, D Gitler, OS Birk
    Nature Communications, 2021-10-26;12(1):6187.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Knockdown of Ptprn-2 delays the onset of puberty in female rats
    Authors: T Kang, J Ye, P Qin, H Li, Z Yao, Y Liu, Y Ling, Y Zhang, T Yu, H Cao, Y Li, J Wang, F Fang
    Theriogenology, 2021-09-28;176(0):137-148.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Viability of diffuse large B-cell lymphoma cells is regulated by kynurenine 3-monooxygenase activity
    Authors: N Morita, M Hoshi, T Hara, S Ninomiya, T Enoki, M Yoneda, H Tsurumi, K Saito
    Oncology Letters, 2021-09-17;22(5):790.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Short lifespan of syngeneic transplanted MSC is a consequence of in vivo apoptosis and immune cell recruitment in mice
    Authors: MB Preda, CA Neculachi, IM Fenyo, AM Vacaru, MA Publik, M Simionescu, A Burlacu
    Cell Death & Disease, 2021-06-02;12(6):566.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. 3-Hydroxykynurenine Regulates Lipopolysaccharide-Stimulated IL-6 Production and Protects against Endotoxic Shock in Mice
    Authors: Masato Hoshi, Hisako Kubo, Tatsuya Ando, Chieko Tashita, Kentaro Nakamoto, Yasuko Yamamoto et al.
    ImmunoHorizons
  13. LRH1 Promotes Tumor Cell Proliferation and Migration and Is Correlated With Poor Prognosis in Ovarian Cancer
    Authors: Wenzhou Sun, Qingtao Shi, Jiaxin Li, Jinmeng Li, Libo Yu
    Frontiers in Oncology
  14. Identification, presence, and possible multifunctional regulatory role of invertebrate gonadotropin-releasing hormone/corazonin molecule in the great pond snail (Lymnaea stagnalis)
    Authors: I Fodor, Z Zrinyi, R Horváth, P Urbán, R Herczeg, G Büki, JM Koene, PS Tsai, Z Pirger
    Gen Comp Endocrinol, 2020-09-20;299(0):113621.
    Species: Snail
    Sample Types: Whole Tissue
    Applications: IHC
  15. Application of the da Vinci surgical robot system in presacral nerve sheath tumor treatment
    Authors: Feifei Pu, Zhicai Zhang, Zhaohui Chen, Kailin Cai, Baichuan Wang, Qiang Wu et al.
    Oncology Letters
  16. Radiation Response of Murine Embryonic Stem Cells
    Authors: CE Hellweg, V Shinde, SP Srinivasan, M Henry, T Rotshteyn, C Baumstark-, C Schmitz, S Feles, LF Spitta, R Hemmersbac, J Hescheler, A Sachinidis
    Cells, 2020-07-09;9(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice
    Authors: C Tashita, M Hoshi, A Hirata, K Nakamoto, T Ando, T Hattori, Y Yamamoto, H Tezuka, H Tomita, A Hara, K Saito
    World J. Gastroenterol., 2020-03-07;26(9):918-932.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Inhibitory effects of genistein in combination with gefitinib on the hepatocellular carcinoma Hep3B cell line
    Authors: Yongxi Tong, Mingshan Wang, Haijun Huang, Jiajie Zhang, Yicheng Huang, Yingjun Chen et al.
    Experimental and Therapeutic Medicine
  19. Telomerase reverse transcriptase ameliorates lung fibrosis by protecting alveolar epithelial cells against senescence
    Authors: T Liu, F Gonzalez D, Y Zhao, Z Wu, AE Rinke, KK Kim, SH Phan
    J. Biol. Chem., 2019-04-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Knockdown of serpin peptidase inhibitor clade C member�1 inhibits the growth of nasopharyngeal carcinoma cells
    Authors: Jin Xu, Yin Ying, Gaoyun Xiong, Liqin Lai, Qingliang Wang, Yue Yang
    Molecular Medicine Reports
  21. Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/ beta -Catenin Pathway
    Authors: Yang Wang, Qi Yang, Yanli Cheng, Meng Gao, Lei Kuang, Chun Wang
    Medical Science Monitor
  22. Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer
    Authors: Guangye Han, Xinjun Zhang, Pei Liu, Quanfeng Yu, Zeyu Li, Qinnan Yu et al.
    International Journal of Oncology
  23. Subcellular Peptide Localization in Single Identified Neurons by Capillary Microsampling Mass Spectrometry
    Authors: L Zhang, N Khattar, I Kemenes, G Kemenes, Z Zrinyi, Z Pirger, A Vertes
    Sci Rep, 2018-08-15;8(1):12227.
    Species: Snail
    Sample Types: Whole Tissue
    Applications: IHC
  24. Age-related up-regulation of carboxyl terminal modulator protein contributes to the decreased brain ischemic tolerance in older rats
    Authors: Jun Li, Weiran Shan, Zhiyi Zuo
    Molecular Neurobiology
  25. Chronic PARP-1 inhibition reduces carotid vessel remodeling and oxidative damage of the dorsal hippocampus in spontaneously hypertensive rats
    Authors: K Eros, K Magyar, L Deres, A Skazel, A Riba, Z Vamos, T Kalai, F Gallyas, B Sumegi, K Toth, R Halmosi
    PLoS ONE, 2017-03-24;12(3):e0174401.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  26. Chronic high fat diet induces cardiac hypertrophy and fibrosis in mice
    Authors: Zhi Wang, Liaoliao Li, Huijuan Zhao, Shuling Peng, Zhiyi Zuo
    Metab. Clin. Exp.
  27. ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons.
    Authors: Shulman Y, Stavsky A, Fedorova T, Mikulincer D, Atias M, Radinsky I, Kahn J, Slutsky I, Gitler D
    J Neurosci, 2015-01-21;35(3):985-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  28. Amantadine Alleviates Postoperative Cognitive Dysfunction Possibly by Increasing Glial Cell Line-derived Neurotrophic Factor in Rats
    Authors: Junfeng Zhang, Hongying Tan, Wei Jiang, Zhiyi Zuo
    Anesthesiology
  29. Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this.
    Authors: Liakhovitskaia A, Rybtsov S, Smith T, Batsivari A, Rybtsova N, Rode C, de Bruijn M, Buchholz F, Gordon-Keylock S, Zhao S, Medvinsky A
    Development, 2014-09-01;141(17):3319-23.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  30. Alteration in the Wnt microenvironment directly regulates molecular events leading to pulmonary senescence.
    Authors: Kovacs T, Csongei V, Feller D, Ernszt D, Smuk G, Sarosi V, Jakab L, Kvell K, Bartis D, Pongracz J
    Aging Cell, 2014-07-01;13(5):838-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  31. Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat.
    Authors: Wang Z, Huang W, Zuo Z
    J Neuroinflammation, 2014-03-25;11(0):56.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  32. Pyrrolidine dithiocarbamate attenuates surgery-induced neuroinflammation and cognitive dysfunction possibly via inhibition of nuclear factor kappa B
    Authors: Junfeng Zhang, Wei Jiang, Zhiyi Zuo
    Neuroscience
  33. Isoflurane Induces Learning Impairment That Is Mediated by Interleukin 1 beta in Rodents
    Authors: Lin Cao, Liaoliao Li, Daowei Lin, Zhiyi Zuo
    PLoS ONE
  34. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012-03-26;92(7):967-77.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. Intercellular transport of Oct4 in mammalian cells: a basic principle to expand a stem cell niche?
    Authors: Rolf HJ, Niebert S, Niebert M, Gaus L, Schliephake H, Wiese KG
    PLoS ONE, 2012-02-16;7(2):e32287.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. 1,25-Dihydroxyvitamin D3 Treatment Delays Cellular Aging in Human Mesenchymal Stem Cells while Maintaining Their Multipotent Capacity
    Authors: Barbara Klotz, Birgit Mentrup, Martina Regensburger, Sabine Zeck, Jutta Schneidereit, Nicole Schupp et al.
    PLoS ONE
  37. Inhibition of exocytosis or endocytosis blocks activity-dependent redistribution of synapsin.
    Authors: Orenbuch A, Ayelet O, Shulman Y, Yoav S, Lipstein N, Noa L, Bechar A, Amit B, Lavy Y, Yotam L, Brumer E, Eliaz B, Vasileva M, Mariya V, Kahn J, Joy K, Barki-Harrington L, Liza BH, Kuner T, Thomas K, Gitler D, Daniel G
    J. Neurochem., 2011-12-02;120(2):248-58.
    Applications: ICC
  38. A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells.
    Authors: Gopalakrishna-Pillai S, Iverson LE
    PLoS ONE, 2011-06-15;6(6):e20663.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Tumor necrosis factor receptor expression and signaling in renal cell carcinoma.
    Authors: Al-Lamki RS, Sadler TJ, Wang J
    Am. J. Pathol., 2010-06-21;177(2):943-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. Standard opioid agonists activate heteromeric opioid receptors: evidence for morphine and [d-Ala(2)-MePhe(4)-Glyol(5)]enkephalin as selective μ-δ agonists
    Authors: Ajay S. Yekkirala, Alexander E. Kalyuzhny, Philip S. Portoghese
    ACS Chemical Neuroscience

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Rabbit IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody
By Anonymous on 01/14/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: RT4-D6P2T rat schwannoma cell line Species: Human

RT4 cell line with Primary snail-rabbit

Immunocytochemistry/Immunofluorescence Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody NL004