Rabbit IgG NorthernLights™ NL637-conjugated Antibody
Rabbit IgG NorthernLights™ NL637-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Rabbit IgG Primary Antibody by Immunocytochemistry. Detection of human Ki-67 by incubating cells with 3 μg/mL of Rabbit anti-Human Ki-67 monoclonal antibody (MAB7617) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated secondary antibody for 1 hour at room temperarture. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).
Reconstitution Calculator
Preparation and Storage
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Rabbit IgG NorthernLights™ NL637-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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The IL-17A-neutrophil axis promotes epithelial cell IL-33 production during nematode lung migration
Authors: Ajendra, J;Papotto, PH;Parkinson, JE;Dodd, RJ;Bombeiro, AL;Pearson, S;Chan, BHK;Ribot, JC;McSorley, HJ;Sutherland, TE;Allen, JE;
Mucosal immunology
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
IL-13 deficiency exacerbates lung damage and impairs epithelial-derived type 2 molecules during nematode infection
Authors: Alistair L Chenery, Silvia Rosini, James E Parkinson, Jesuthas Ajendra, Jeremy A Herrera, Craig Lawless et al.
Life Science Alliance
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A micro-sterile inflammation array as an adjuvant for influenza vaccines.
Authors: Wang J, Shah D, Chen X, Anderson R, Wu M
Nat Commun, 2014-07-18;5(0):4447.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
mTORC1 and mTORC2 play different roles in the functional survival of transplanted adipose-derived stromal cells in hind limb ischemic mice via regulating inflammation in vivo.
Authors: Fan W, Cheng K, Qin X, Narsinh K, Wang S, Hu S, Wang Y, Chen Y, Wu J, Xiong L, Cao F
Stem Cells, 2013-01-01;31(1):203-14.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
FAQs
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Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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