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Name: Sheep IgG NorthernLights™ NL493-conjugated Antibody
Brand: R&D Systems
SKU: NL012
Rating: 3 (1 reviews)

All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

Sheep IgG NorthernLights™ NL493-conjugated Antibody

Catalog #: NL012
6 Citations 1 Review Datasheet / COA / SDS

NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed

Catalog #	Availability	Size / Price	Qty
NL012

Detection of Sheep IgG Primary Antibody by Immunocytochemistry.

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Product Details

Citations (6)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Sheep IgG NorthernLights™ NL493-conjugated Antibody Summary

Species Reactivity

Sheep

Specificity

Sheep IgG (H+L)

Source

Polyclonal Donkey IgG

Purification

Antigen Affinity-purified

Immunogen

Sheep IgG

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Label

NL493 (Excitation= 493 nm, Emission= 514 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Use at a 1:200 dilution

Immunohistochemistry

Use at a 1:200 dilution

Immunocytochemistry

Use at a 1:200 dilution

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Sheep IgG Primary Antibody by Immunocytochemistry. Detection of human AIF by incubating cells with 5 μg/mL of Sheep anti-Human AIF polyclonal antibody (AF5824) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in KG-1 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Product Datasheet

COA

SDS

Citations for Sheep IgG NorthernLights™ NL493-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells
Authors: Ryoo, H;Underhill, GH;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: ICC
Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays
Authors: MS Szymczak, WK Reeves, MM Miller
Viruses, 2021-08-30;13(9):.
Species: Cercopithecus aethiops
Sample Types: Whole Cells
Applications: ICC
Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix
Authors: T Kryza, C Parent, J Pardessus, A Petit, J Burlaud-Ga, P Reverdiau, S Iochmann, V Labas, Y Courty, N Heuzé-Vour
Sci Rep, 2018-04-20;8(1):6331.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Inhibition of VEGF-dependent angiogenesis and tumor angiogenesis by an optimized antibody targeting CLEC14a
Authors: TK Kim, CS Park, J Jang, MR Kim, HJ Na, K Lee, HJ Kim, K Heo, BC Yoo, YM Kim, JW Lee, SJ Kim, ES Kim, DY Kim, K Cha, TG Lee, S Lee
Mol Oncol, 2018-01-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro.
Authors: Ki, M K, Jeoung, M H, Choi, J R, Rho, S-S, Kwon, Y-G, Shim, H, Chung, J, Hong, H J, Song, B D, Lee, S
Oncogene, 2013-05-06;32(48):5449-57.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
HIV-1 harboring renal tubular epithelial cell interaction with T cells results in T cell trans-infection.
Authors: Mikulak J, Teichberg S, Faust T, Schmidtmayerova H, Singhal PC
Virology, 2008-12-17;385(1):105-14.
Species: Human
Sample Types: Whole Cells
Applications: ICC

FAQs

Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

View all Antibody FAQs