dTAG-Fluorescein
Chemical Name: (R)-1-(2-(2-((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)amino)-2-oxoethoxy)phenyl)-3-(3,4-dimethoxyphenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
Purity: ≥95%
Biological Activity
dTAG-Fluorescein is a fluorescent probe for labeling FKBP12F36V fusion proteins. Comprises a high-affinity ligand for FKBP12F36V mutant protein (Kd in the subnanomolar range and 1,000-fold selectivity for the F36V mutant of FKBP12 over wild-type) coupled to 5-amino fluorescein. dTAG-Fluorescein can fluorescently label FKBP12F36V fusion proteins expressed at different levels in a variety of cell lines.Optical Data for dTAG-Fluorescein
| Emission Color | Green |
|---|---|
| λabs | 493 nm |
| λem | 514 nm |
| Extinction Coefficient (ε) | 68800 M-1cm-1 |
| Closest Laser line | 488 nm |
| Application | Flow cytometry, Confocal microscopy |
Plan Your Experiments
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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The dTAG system for immediate and target-specific protein degradation.
Nabet et al.
Nat.Chem.Biol., 2018;14:431
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