DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody
DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of DYKDDDDK Epitope Tag in HEK293 cells by Flow Cytometry. HEK293 cells transfected with DYKDDDDK-tagged proteins (filled histogram) vs Irrelevant HEK293 wild type cells (open histogram) were stained with Rabbit Anti-DYKDDDDK Epitope Tag Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8529G) at 0.001 ug/106 cells. To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Product Datasheets
Product Specific Notices
FLAG® is a registered trademark of Sigma-Aldrich Co. LLC
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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T cells selectively filter oscillatory signals on the minutes timescale
Authors: Geoff P. O’Donoghue, Lukasz J. Bugaj, Warren Anderson, Kyle G. Daniels, David J. Rawlings, Wendell A. Lim
Proceedings of the National Academy of Sciences
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Structural basis for the ligand recognition and G protein subtype selectivity of kisspeptin receptor
Authors: Wu, Z;Chen, G;Qiu, C;Yan, X;Xu, L;Jiang, S;Xu, J;Han, R;Shi, T;Liu, Y;Gao, W;Wang, Q;Li, J;Ye, F;Pan, X;Zhang, Z;Ning, P;Zhang, B;Chen, J;Du, Y;
Science advances
Species: Human
Sample Types: Transfected Whole Cells
Applications: Flow Cytometry -
Degron-based bioPROTACs for controlling signaling in CAR T cells
Authors: Kim, MS;Bhargava, HK;Shavey, GE;Lim, WA;El-Samad, H;Ng, AH;
bioRxiv : the preprint server for biology
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesicles
Authors: Mitrut, RE;Stranford, DM;Chan, JM;Bailey, MD;Luo, M;Meade, TJ;Wang, M;Leonard, JN;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Extracellular Vesicles, Whole Cells
Applications: Flow Cytometry -
ADR3, a next generation i-body to human RANKL, inhibits osteoclast formation and bone resorption
Authors: H Qiu, C Hosking, E Rothzerg, A Samantha, K Chen, V Kuek, H Jin, S Zhu, A Vrielink, K Lim, M Foley, J Xu
The Journal of Biological Chemistry, 2023-01-09;0(0):102889.
Species: Human
Sample Types: Whole Cells
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Programming Multicellular Assembly with Synthetic Cell Adhesion Molecules
Authors: AJ Stevens, AR Harris, J Gerdts, KH Kim, C Trentesaux, JT Ramirez, WL McKeithan, F Fattahi, OD Klein, DA Fletcher, WA Lim
Nature, 2022-12-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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