DYKDDDDK Epitope Tag Antibody
DYKDDDDK Epitope Tag Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1, N-terminal DYKDDDDK-tagged UCHL5, and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa, 40 kDa, 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of DYKDDDDK (FLAG®) Epitope Tag by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20, loaded at 0.1 mg/mL. Specific band were detected for DYKDDDDK-tagged proteins at approximately 64 and 136 kDa (as indicated) using 1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of DYKDDDDK Epitope Tag in HEK293 cells transfected with FLAG vs Irrelevant HEK293 wild type cells by Flow Cytometry. HEK293 cells transfected with DYKDDDDK-tagged proteins (filled histogram) vs Irrelevant HEK293 wild type cells (open histogram) were stained with Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) at 0.001 ug/106 cells followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Product Datasheets
Citations for DYKDDDDK Epitope Tag Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Identification of the Functional Autophagy-Regulatory Domain in HCLS1-Associated Protein X-1 That Resists Against Oxidative Stress.
Authors: Li YL, Cai WF, Wang L et al.
DNA Cell Biol
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N-glycosylation status of Trop2 impacts its surface density, interaction with claudin-7 and exosomal release
Authors: PR Kamble, SR Patkar, AA Breed, BR Pathak
Archives of biochemistry and biophysics, 2021-11-10;714(0):109084.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models
Authors: WH Liu, K Völse, D Senft, I Jeremias
Scientific Reports, 2021-06-16;11(1):12649.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner
Authors: W Zhang, G Yin, H Zhao, H Ling, Z Xie, C Xiao, Y Chen, Y Lin, T Jiang, S Jin, J Wang, X Yang
Cell Death & Disease, 2021-01-20;12(1):102.
Species: Human
Sample Types: Cell Lystates
Applications: Immunoprecipitation
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