Feline Fas/TNFRSF6/CD95 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Fas/TNFRSF6/CD95

Feline Fas (fibroblast associated; also named CD95 and APO-1) is a 45 kDa type I transmembrane (TM) glycoprotein that is a member of the TNF receptor superfamily, designated TNFRSF6 (1-3). The family contains about 30 members, and is characterized by the presence of at least one cysteine-rich domain that contains multiple intrachain disulfide bonds. In general, the superfamily is divided into cytoplasmic death domain (DD) containing, and non-DD containing receptors (3). Feline Fas is synthesized as a 314 amino acid (aa) precursor that contains a 24 aa signal sequence, a 148 aa extracellular region, a 16 aa TM segment, and a 126 aa cytoplasmic tail (4). The extracellular region contains four potential N-linked glycosylation sites plus two distinct cysteine-rich domains of approximately 40 aa each; the cytoplasmic tail shows a 45 aa DD. The extracellular region of feline Fas shares 68%, 65%, 53%, and 58% aa sequence identity to porcine, human, mouse, and rat Fas, respectively. There are five alternate splice forms of feline Fas, which vary from 132 aa to 209 aa in length. All use exons 1-3 (aa 1-111) and all are missing the transmembrane segment of the full length form (5). Circulating Fas is reported to be both a dimer and trimer at low ng/mL concentrations. The ligand for Fas is FasL, and Fas ligation activates both the MEK cascade and FADD/caspase-8 pathway (7).

Product Datasheets

Related Research Areas

• Adipocytokines
• Alpha Granule Soluble Molecules
• B Cell Development and Differentiation
• Cytokine and Growth Factor Receptors on VSMC
• gamma delta T Cells
• Immune Regulatory Molecules on Endothelial Cells
• Isolation, Culture, and Identification of Natural Killer Cells
• Leukocyte Receptors/Markers in Tfh Cell Differentiation and Function
• Regulation of Apoptosis by TNF Superfamily Members
• Regulatory T Cells (Tregs)
• TNF Superfamily
• TNF Superfamily Receptors