Feline IFN-gamma Antibody

Catalog #: AF764
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF764
AF764-SP

IFN‑ gamma Inhibition of VSV-induced Cytopathy and Neutralization by Feline IFN‑ gamma Antibody.

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Feline IFN-gamma Antibody Summary

Species Reactivity

Feline

Specificity

Detects feline IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human, mouse, rat, and porcine IFN-gamma is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant feline IFN-gamma
Gln24-Lys167
Accession # P46402

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Feline IFN-gamma (Catalog # 764-FG)

Neutralization

Measured by its ability to neutralize IFN‑ gamma inhibition of VSV-induced cytopathy in the CRFK feline kidney epithelial cell line. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 5 ng/mL Recombinant Feline IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IFN‑ gamma Inhibition of VSV-induced Cytopathy and Neutralization by Feline IFN‑ gamma Antibody. Recombinant Feline IFN-gamma (Catalog # 764-FG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the CRFK feline kidney epithelial cell line in a dose-dependent manner (orange line). Inhibition of VSV activity elicited by Recombinant Feline IFN-gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF764). The ND₅₀ is typically 1-3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature feline IFN-gamma exists as a noncovalently linked homodimer of 20 25 kDa variably glycosylated subunits (3, 4). It shares 88% amino acid sequence identity with canine IFN-gamma, 72%‑78% with bovine, equine, and porcine IFN‑ gamma, and 40%‑62% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8).

References

Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
Pestka, S. et al. (2004) Immunol. Rev. 202:8.
Argyle, D.J. et al. (1995) DNA Seq. 5:169.
Schijns, V.E. et al. (1995) Immunogenetics 42:440.
Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Citation for Feline IFN-gamma Antibody

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