FITC
Chemical Name: 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity: ≥95%
Biological Activity
Key information: FITC (an amine-reactive version of the parent dye, fluorescein) is a widely-used green fluorescent dye supplied with an isothiocyanate reactive group for the labeling of primary amines. FITC is bright and has good water-solubility, however it is sensitive to changes in pH and temperature, and has a relatively high photobleaching rate.Application: Suitable for flow cytometry, immunofluorescence (IHC, ICC), fluorescence microscopy, fluorescence in situ hybridization (FISH), biochemical assays.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 495 nm and 525 nm, respectively; quantum yield = 0.92; extinction coefficient = 75,000 M-1cm-1; A280 = 0.35.
Optical Data for FITC
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 495 nm |
| λem | 525 nm |
| Extinction Coefficient (ε) | 75000 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Reactive Group | Isothiocyanate |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.35 |
| Application | Flow Cytometry, Immunofluorescence (IHC, ICC), Fluorescence Microscopy, FISH |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays.
Yang et al.
Biochemistry, 2014;53:4881
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Citation for FITC
The citations listed below are publications that use Tocris products. Selected citations for FITC include:
1 Citation: Showing 1 - 1
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Lipidic nanocapsule drug delivery: neuronal protection for cochlear implant optimization.
Authors: Meyer Et al.
Int J Nanomedicine 2012;7:2449
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