Home / Calcium Signaling Probes and Ca2+ Indicator Dyes / Fluo-4 AM

Fluo-4 AM

Catalog #: 6255
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6255/1
Fluo-4 AM | CAS No. 273221-67-3 | Calcium Signaling Probes and Ca2+ Indicator Dyes
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Description: Cell-permeable, fluorescent Ca2+ indicator

Chemical Name: N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester

Purity: ≥95%

Product Details
Citations (2)
Reviews
Biological Activity Technical Data Background Product Datasheets Calculators

Biological Activity

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.

It is recommended to prepare stock solutions in DMSO.

Optical Data for Fluo-4 AM

Emission Color Green
λabs 494 nm
λem 506 nm
Closest Laser line 488 nm
Cell Permeable Yes
Application Fluorescence Microscopy, Flow Cytometry, Fluorometric Microplate Reading Assays

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
1096.95
Formula:
C51H50F2N2O23
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
273221-67-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes.
    Gee et al.
    Cell Calcium., 2000;27:97

Product Datasheets

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Citations for Fluo-4 AM

The citations listed below are publications that use Tocris products. Selected citations for Fluo-4 AM include:

2 Citations: Showing 1 - 2

  1. Norepinephrine transporter defects lead to sympathetic hyperactivity in Familial Dysautonomia models.
    Authors: Gerald W Et al.
    Nat Commun  2022;13:7032
  2. Heterosynaptic Plasticity Determines the Set Point for Cortical Excitatory-Inhibitory Balance.
    Authors: Bernardo Et al.
    Neuron  2020;106:842-854.e4

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