Flutax 2
Chemical Name: (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(2',7'-difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine
Purity: ≥90%
Biological Activity
Key information: Flutax 2 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions.
Application: fluorescence microscopy.
Properties and Photophysical Data: The fluorescent label on Flutax 2 is attached by derivatization of the 7-β-hydroxy group of native paclitaxel which permits selective binding of the probe to microtubules. Flutax 2 binds microtubules with high affinity (Ka ~ 107M-1). Flutax 2 binds to α, β tubulin dimers and stabilizes them. Flutax 2 is soluble and pH insensitive and is more photostable than Flutax 1 (Cat. No. 2226). Excitation and emission maxima (λ) are 496 nm and 526 nm, respectively.
Scientific Data
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Flutax 2 labeled HeLa cells. Flutax 2 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 2 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 2 staining is not retained at all after fixation (photobleach). Like Flutax 1, Flutax 2 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good signal.
Optical Data for Flutax 2
| λabs | 496 nm |
|---|---|
| λem | 526 nm |
| Extinction Coefficient (ε) | 80000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Application | Fluorescence Microscopy |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Location and properties of the Tax. binding center in microtubules: a picosecond laser study with fluorescent taxoids.
Lillo et al.
Biochemistry., 2002;41:12436 -
Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site.
Diaz et al.
J.Biol.Chem., 2000;275:26265
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