GFP APC-conjugated Antibody Summary
Ser2-Lys238
Accession # P42212
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of GFP in HEK293 Human Cell Line Transfected with GFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) wild-type GFP or (B) eGFP was stained with Goat Anti-GFP APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC4240A). Quadrant markers were set based on control antibody staining (Catalog # IC108A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GFP
Green Fluorescent Protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.
Product Datasheets
Citation for GFP APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells
Authors: Y Diao, R Fang, B Li, Z Meng, J Yu, Y Qiu, KC Lin, H Huang, T Liu, RJ Marina, I Jung, Y Shen, KL Guan, B Ren
Nat. Methods, 2017-04-17;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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