GFP PE-conjugated Antibody

Catalog #: IC42401P Datasheet / COA / SDS

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IC42401P

Detection of GFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry.

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All GFP Antibodies

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GFP PE-conjugated Antibody Summary

Specificity

Detects recombinant GFPuv and eGFP in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 454505

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant GFPuv
Ser2-Lys238
Accession # P42212

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of GFP antibody in HEK293 Human Cell Line Transfected with eGFP antibody by Flow Cytometry.

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Detection of GFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP was stained with either (A) Mouse Anti-GFP PE-conjugated Monoclonal Antibody (Catalog # IC42401P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

Long Name

Green Fluorescent Protein

Alternate Names

eGFP; Enhanced Green Fluorescent Protein; GFP; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein