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Glutathione S-Transferase/GST Antibody

Catalog #: MAB94991 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB94991-100
MAB94991-SP
Detection of Glutathione S‑Transferase/GST by Western Blot.
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Glutathione S-Transferase/GST Antibody Summary

Specificity
Detects Glutathione S‑Transferase/GST in direct ELISAs.
Source
Monoclonal Hamster IgG Clone # PIP-1D7-54.3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
GST-tagged recombinant protein
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Glutathione S-Transferase/GST antibody by Western Blot. View Larger

Detection of Glutathione S‑Transferase/GST by Western Blot. Western blot shows lysates ofSf 21 S. frugiperdainsect ovarian cell line mock transfected or transfected GST tagged Parkin. PVDF membrane was probed with 1 µg/mL of Hamster Anti-Glutathione S‑Transferase/GST Monoclonal Antibody (Catalog # MAB94991) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for Glutathione S‑Transferase/GST tagged Parkin at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase/GST

Entrez Gene IDs
2950 (Human)
Alternate Names
Glutathione STransferase; Glutathione S-Transferase

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