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Mouse IgG Antibody

Catalog #: AF007
17 Citations Datasheet / COA / SDS
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AF007
R&D Systems Antibodies
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Citations (17)
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Mouse IgG Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG1, IgG2A, IgG2B, IgG3 heavy and light chains.  In direct ELISAs, less than 15% cross-reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot

Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Mouse IgG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis
    Authors: ShengPeng Wang, Bianbian Wang, Yue Shi, Tanja Möller, Rebekka I. Stegmeyer, Boris Strilic et al.
    Blood
  2. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease
    Authors: Mansky RH, Greguske EA, Yu D et al.
    Cell reports
  3. Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice
    Authors: N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou
    Frontiers in Physiology, 2023-01-04;13(0):1061715.
    Species: Mouse, N/A
    Sample Types: Protein, Recombinant Protein
    Applications: Western Blot
  4. Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway
    Authors: N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada
    Journal of dental sciences, 2023;18(2):567-576.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway
    Authors: N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada
    Journal of dental sciences, 2022-09-16;18(2):567-576.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Boosting immune surveillance by low-dose PI3K inhibitor facilitates early intervention of breast cancer
    Authors: J Wang, Y Zhang, Y Xiao, X Yuan, P Li, X Wang, Y Duan, VL Seewaldt, D Yu
    American journal of cancer research, 2021-05-15;11(5):2005-2024.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization Control
  7. Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer
    Authors: S Wu, M Luo, KKW To, J Zhang, C Su, H Zhang, S An, F Wang, D Chen, L Fu
    Molecular Cancer, 2021-01-18;20(1):17.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  8. Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling
    Authors: HA Himburg, M Roos, T Fang, Y Zhang, CM Termini, L Schlussel, MM Kim, A Pang, J Kan, L Zhao, H Suh, JP Sasine, G Sapparapu, PM Bowers, G Schiller, JP Chute
    J. Clin. Invest., 2020-01-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Autoreactive CD19+CD20- Plasma Cells Contribute to Disease Severity of Experimental Autoimmune Encephalomyelitis.
    Authors: Chen D, Ireland S, Davis L, Kong X, Stowe A, Wang Y, White W, Herbst R, Monson N
    J Immunol, 2016-01-13;196(4):1541-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  10. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2014-10-22;515(7525):130-3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Vacuolar-ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis.
    Authors: Jaiswal M, Katara G, Mallers T, Chaouat G, Gilman-Sachs A, Beaman K
    J Leukoc Biol, 2014-03-12;96(2):337-47.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Absence of a role for interleukin-13 in inflammatory bowel disease.
    Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
    Eur J Immunol, 2014-02-01;44(2):370-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  13. Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
    Authors: Li N, Mao D, Lu S, Tong C, Zhang Y, Long M
    J Immunol, 2013-03-20;190(8):4371-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure.
    Authors: Westenbrink BD, Ruifrok WP, Voors AA
    Cardiovasc. Res., 2010-02-05;87(1):30-9.
    Species: Rat
    Sample Types: In Vivo
    Applications: Control
  15. IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice
    Authors: RO Bello, MA Abdullah, R Abd Majid, VK Chin, MF Abd Rachma, ZO Ibraheem, MK Hussain, MG Magaji, R Basir
    Malar. J., 2019-12-19;18(1):434.
  16. Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method
    Authors: Mohammad Rahbar, Yuling Wu, J. Anand Subramony, Guozhen Liu
    Frontiers in Bioengineering and Biotechnology
  17. Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
    Authors: Peijun J. W. He, Ioannis N. Katis, Robert W. Eason, Collin L. Sones
    Biosensors (Basel)

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