Home / IgG / Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Catalog #: F0115
2 Citations Datasheet / COA / SDS

Discontinued Product

F0115 has been discontinued.
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG heavy and light chains
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rat origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TnP Peptide Suppresses Experimental Autoimmune Encephalomyelitis (EAE) in a Preclinical Mouse Model
    Authors: Carla Lima, Adolfo Luis Almeida Maleski, Jefferson Thiago Gonçalves Bernardo, Vitor Cataldi Zelli, Evilin Naname Komegae, Monica Lopes-Ferreira
    Frontiers in Immunology
  2. Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis
    Authors: EN Komegae, TA Souza, LZ Grund, C Lima, M Lopes-Ferr
    PLoS ONE, 2017-02-24;12(2):e0171796.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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