Home / IgG / Goat IgG VisUCyte HRP Polymer

Goat IgG VisUCyte HRP Polymer

Catalog #: VC004
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
VC004-025
VC004-050
VC004-125
VC004-250
Detection of HAI-1 in Human Lung Cancer.
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Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat IgG VisUCyte HRP Polymer Summary

Species Reactivity
Goat
Specificity
Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied as a solution in PBS with preservative.
Label
HRP Polymer(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Immunohistochemistry
Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Detection of HAI-1 in Human Lung Cancer. View Larger

Detection of HAI-1 in Human Lung Cancer. HAI-1 was detected in paraffin-embedded sections of human lung cancer using Goat Anti-Human HAI-1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 1 µg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat IgG VisUCyte HRP Polymer

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. HAPLN1 potentiates peritoneal metastasis in pancreatic cancer
    Authors: L Wiedmann, F De Angelis, N Vaquero-Si, E Donato, E Espinet, I Moll, E Alsina-San, H Bohnenberg, E Fernandez-, R Mülfarth, M Vacca, J Gerwing, LC Conradi, P Ströbel, A Trumpp, C Mogler, A Fischer, J Rodriguez-
    Nature Communications, 2023-04-24;14(1):2353.
    Species: Goat
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Live-attenuated pediatric parainfluenza vaccine expressing 6P-stabilized SARS-CoV-2 spike protein is protective against SARS-CoV-2 variants in hamsters
    Authors: X Liu, HS Park, Y Matsuoka, C Santos, L Yang, C Luongo, IN Moore, RF Johnson, NL Garza, P Zhang, P Lusso, SM Best, UJ Buchholz, CL Nouën
    bioRxiv : the preprint server for biology, 2022-12-13;0(0):.
    Species: Hamster
    Sample Types: Whole Tissue
    Applications: IHC
  3. Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection
    Authors: JR Sheldon, LE Himmel, DE Kunkle, AJ Monteith, KN Maloney, EP Skaar
    PloS Pathogens, 2022-09-02;18(9):e1010809.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis
    Authors: Y Yamada, Y Zhong, S Miki, A Taura, TH Rabbitts
    Scientific Reports, 2022-05-04;12(1):7226.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. SARS-CoV-2 entry sites are present in all structural elements of the human glossopharyngeal and vagal nerves: Clinical implications
    Authors: L Vitale-Cro, I Szalayova, A Scoggins, M Palkovits, E Mezey
    EBioMedicine, 2022-04-04;78(0):103981.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma
    Authors: C Nadolny, X Zhang, Q Chen, SF Hashmi, W Ali, C Hemme, N Ahsan, Y Chen, R Deng
    American journal of cancer research, 2021-10-15;11(10):4746-4767.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. CLEC3A, MMP7, and LCN2 as novel markers for predicting recurrence in resected G1 and G2 pancreatic neuroendocrine tumors
    Authors: M Miki, T Oono, N Fujimori, T Takaoka, K Kawabe, Y Miyasaka, T Ohtsuka, D Saito, M Nakamura, Y Ohkawa, Y Oda, M Suyama, T Ito, Y Ogawa
    Cancer Med, 2019-05-25;0(0):.
    Species: Human
    Sample Types: Whole Tissue
  8. Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients
    Authors: H Hu, Z Liu, C Liu
    Oncol Lett, 2019-01-07;17(3):2788-2794.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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