H/M Pluripotent Stem Cell Multi-Color Flow Cytometry Kit
H/M Pluripotent Stem Cell Multi-Color Flow Cytometry Kit Summary
Kit Summary
Specifications
Product Datasheets
Customers also Viewed
Scientific Data
![Verification of Human BG01V Embryonic Stem Cell Pluripotency by Multi-Color Flow Cytometry. Verification of Human BG01V Embryonic Stem Cell Pluripotency by Multi-Color Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/fmc001_h-m-pluripotent-stem-cell-multi-color-flow-cytometry-kit-2532021113420.png)
Verification of Human BG01V Embryonic Stem Cell Pluripotency by Multi-Color Flow Cytometry. BG01V human embryonic stem cells were stained using reagents included in the Human/Mouse Pluripotent Stem Cell Multi-Color Flow Cytometry Kit (Catalog # FMC001). Cells were simultaneously analyzed for expression of pluripotent markers including SSEA-1, SSEA-4, Oct-3/4, and SOX2 by flow cytometry. A. Flow cytometry data shows that 91.9% of BG01V human embryonic stem cells are positive for both Oct-3/4 and SSEA4 expression. B. Flow cytometry data shows that 88.5% of BG01V human embryonic stem cells are positive for SSEA-4 and negative for SSEA-1, a phenotype consistent with human embryonic stem cells. C. Flow cytometric analysis shows that BG01V human embryonic stem cells express the pluripotent marker SOX2.
![Verification of Mouse D3 Embryonic Stem Cell Pluripotency by Multi-Color Flow Cytometry. Verification of Mouse D3 Embryonic Stem Cell Pluripotency by Multi-Color Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/fmc001_h-m-pluripotent-stem-cell-multi-color-flow-cytometry-kit-253202111355.png)
Verification of Mouse D3 Embryonic Stem Cell Pluripotency by Multi-Color Flow Cytometry. Mouse D3 embryonic stem cells were stained using reagents included in the Human/Mouse Embryonic Stem Cell Multi-Color Flow Cytometry Kit (Catalog # FMC001). Cells were analyzed for expression of pluripotent markers including SSEA-1, SSEA-4, Oct-3/4, and SOX2 by flow cytometry. A. Flow cytometric analysis shows that 91.1% of mouse D3 embryonic stem cells are positive for both Oct-3/4 and SSEA1 expression. B. Flow cytometric analysis data shows that 82.6% of mouse D3 embryonic stem cells are positive for SSEA-1 and negative for SSEA-4 a phenotype consistent with mouse embryonic stem cells. C. Flow cytometric analysis shows that mouse D3 embryonic stem cells express the pluripotent marker SOX2.
Assay Procedure
>Refer to the product datasheet for complete product details.
Briefly, stem cell pluripotency is assessed by flow cytometry using the following procedure:
- Fix and permeabilize cells using Fixation/Permeabilization Buffer
- Stain cells with fluorochrome-conjugated antibodies or isotype controls
- Wash and resuspend cells in PBS
- Analyze samples by flow cytometry
Reagents Provided
Reagents Supplied in the Human/Mouse Embryonic Stem Cell Multi-Color Flow Cytometry Kit (Catalog # FMC001)
- SOX2-PE (Clone 245610; mouse IgG2A)
- Oct-3/4-APC (Clone 240408; rat IgG2B)
- SSEA-1-PerCP (Clone MC-480; mouse IgM)
- SSEA-4-CFS (Clone MC-813-70; mouse IgG3)
- Mouse IgM Isotype Control
- Mouse IgG3 Isotype Control
- Rat IgG2B Isotype Control
- Mouse IgG2A Isotype Control
- Fixation/Permeabilization Buffer (with 1% formaldehyde, 8.9% saponin, and <0.05% sodium azide)
- Permeabilization/Wash Buffer (with 10% saponin and 0.05% sodium azide)
- Includes enough reagents to perform 25 assays
Other Supplies Required
- PBS or Hanks’ Balanced Salt Solution (HBSS)
Procedure Overview
Intracellular Staining Protocol with Simultaneous Fixation/Permeabilization
- Harvest cells and wash 2 times with PBS or HBSS.
![wash with PBS or HBSS](http://resources.rndsystems.com/images/site/fmc001-protocol-step1.png)
- Resuspend cells in Fixation/Permeabilization Buffer (approximately 5 x 105 cells/0.5 mL) and transfer to 5 mL flow cytometry tubes.
- Incubate cells at room temperature for 30 minutes, vortexing intermittently.
![resuspend cells in buffer and transfer to flow cytometry tubes](http://resources.rndsystems.com/images/site/fmc001-protocol-steps2and3.png)
- Centrifuge samples at 300 x g for 5 minutes.
- Resuspend the pellet in 100-200 μL of Permeabilization/Wash Buffer.
![resuspend the pellet in wash buffer](http://resources.rndsystems.com/images/site/fmc001-protocol-steps2and3.png)
- Add 10 µL of each fluorochrome-conjugated antibody or the corresponding isotype control antibody.
- Incubate the samples at room temperature for 30-40 minutes in the dark.
![add fluorchorome-conjugated antibody or isotype control antibody](http://resources.rndsystems.com/images/site/fmc001-protocol-step4.png)
- Wash the cells in Permeabilization/Wash Buffer.
- Resuspend the cells in 200-400 μL of PBS.
![wash cells in buffer and resuspend cells in PBS](http://resources.rndsystems.com/images/site/fmc001-protocol-step5.png)
- Analyze the expression of pluripotent markers simultaneously by flow cytometry.
![Analyze the expression of pluripotent markers simultaneously by flow cytometry](http://resources.rndsystems.com/images/site/fmc001-protocol-step6.png)
Citations for H/M Pluripotent Stem Cell Multi-Color Flow Cytometry Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Generation of the human pluripotent stem cell lines KUMi005-A from a patients with multiple myeloma
Authors: JH Kim, JH Hong, KW Kang, BH Lee, Y Park, BS Kim
Stem Cell Research, 2022-10-13;65(0):102939. 2022-10-13
-
Generation of human induced iPSC KUMi003-A from acute promyelocytic leukemia (APL) M3
Authors: JH Kim, KW Kang, BH Lee, Y Park, BS Kim
Oncogene, 2022-07-09;63(0):102861. 2022-07-09
-
Generation of a human induced pluripotent stem cell line KUMi006 from a patient with multiple myeloma
Authors: JH Hong, JH Kim, KW Kang, BH Lee, Y Park, BS Kim
Stem Cell Research, 2022-03-28;61(0):102767. 2022-03-28
-
Generation and characterization of a human iPSC line SANi006-A from a Gray Platelet Syndrome patient
Authors: CEM Aarts, E Varga, S Webbers, J Geissler, M von Linder, TW Kuijpers, E van den Ak
Stem Cell Research, 2021-06-29;55(0):102443. 2021-06-29
-
Generation and characterization of a human iPSC line SANi007-A from a patient with a heterozygous dominant mutation in ELANE
Authors: CEM Aarts, E Varga, S Webbers, J Geissler, M von Linder, TW Kuijpers, E van den Ak
Stem Cell Research, 2021-06-27;55(0):102440. 2021-06-27
-
Generation and characterization of a human iPSC line SANi008-A from a Ch�diak-Higashi Syndrome patient
Authors: CEM Aarts, E Varga, S Webbers, J Geissler, M von Linder, TW Kuijpers, E van den Ak
Stem Cell Research, 2021-06-24;55(0):102442. 2021-06-24
-
Generation and characterization of a control and patient-derived human iPSC line containing the Hermansky Pudlak type 2 (HPS2) associated heterozygous compound mutation in AP3B1
Authors: CEM Aarts, E Karampini, T Wüst, S Webbers, E Varga, J Geissler, J Voorberg, M von Linder, R Bierings, E van den Ak, TW Kuijpers
Stem Cell Research, 2021-06-23;54(0):102444. 2021-06-23
-
Modelling heme-mediated brain injury associated with cerebral malaria in human brain cortical organoids
Authors: A Harbuzariu, S Pitts, JC Cespedes, KO Harp, A Nti, AP Shaw, M Liu, JK Stiles
Sci Rep, 2019-12-16;9(1):19162. 2019-12-16
-
Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line
Authors: N Malerba, P Benzoni, GM Squeo, R Milanesi, F Giannetti, LG Sadleir, G Poke, B Augello, AI Croce, A Barbuti, G Merla
Stem Cell Res, 2019-08-22;40(0):101547. 2019-08-22
-
Efficient Generation of Non-Integration and Feeder-Free Induced Pluripotent Stem Cells from Human Peripheral Blood Cells by Sendai Virus
Authors: H Ye, Q Wang
Cell. Physiol. Biochem., 2018-10-24;50(4):1318-1331. 2018-10-24
-
Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50
Authors: B Ermon, CB Volpato, G Cattelan, R Silipigni, M Di Segni, C Cantaloni, M Casella, PP Pramstalle, G Pompilio, E Sommariva, V Meraviglia, A Rossini
Stem Cell Res, 2018-09-06;32(0):78-82. 2018-09-06
-
Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
Sci Rep, 2016-08-10;6(0):31063. 2016-08-10
-
Differentiation and transplantation of functional pancreatic beta cells generated from induced pluripotent stem cells derived from a type 1 diabetes mouse model.
Authors: Jeon, Kilsoo, Lim, Hyejin, Kim, Jung-Hyu, Thuan, Nguyen V, Park, Seung Hw, Lim, Yu-Mi, Choi, Hye-Yeon, Lee, Eung-Ryo, Kim, Jin-Hoi, Lee, Myung-Sh, Cho, Ssang-Go
Stem Cells Dev, 2012-06-01;21(14):2642-55. 2012-06-01
FAQs
No product specific FAQs exist for this product, however you may
View all Flow Cytometry Kit FAQsReviews for H/M Pluripotent Stem Cell Multi-Color Flow Cytometry Kit
There are currently no reviews for this product. Be the first to review H/M Pluripotent Stem Cell Multi-Color Flow Cytometry Kit and earn rewards!
Have you used H/M Pluripotent Stem Cell Multi-Color Flow Cytometry Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image