HA Tag Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC6875R-025
IC6875R-100
Detection of HA Tag in HEK293 Human Cell Line Transfected with HA-tagged Proteins by Flow Cytometry.
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Product Details
Citations (4)
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HA Tag Alexa Fluor® 647-conjugated Antibody Summary

Specificity
Stains N-terminal,C-terminal, and Internal Sequence HA-tagged protein transfectants but not irrelevant transfectants in flow cytometry.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 912426
Purification
Protein A or G purified from cell culture supernatant
Immunogen
HA tag peptide
Accession # ABB51961
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of HA Tag antibody in HEK293 Human Cell Line Transfected with HA-tagged Proteins antibody by Flow Cytometry. View Larger

Detection of HA Tag in HEK293 Human Cell Line Transfected with HA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) HA-tagged proteins (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Mouse Anti-HA Tag Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC6875R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HA Tag

Entrez Gene IDs
23308115 (Influenza A Virus H1N1); 3655151 (Influenza A Virus H3N2)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for HA Tag Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CD19/CD22 bispecific chimeric antigen receptor?NK?92 cells are developed and evaluated
    Authors: Kim, H;Han, M;Kim, M;Kim, H;Im, HJ;Kim, N;Koh, KN;
    Oncology letters
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Oncogenic BRAF induces whole-genome doubling through suppression of cytokinesis
    Authors: R Darp, MA Vittoria, NJ Ganem, CJ Ceol
    Oncogene, 2022-07-15;13(1):4109.
    Species: Zebrafish
    Sample Types: Whole Cells
    Applications: ICC
  3. Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone
    Authors: A Diane, N Mahmoud, I Bensmail, N Khattab, HA Abunada, M Dehbi
    Sci Rep, 2020-11-24;10(1):20482.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Feasibility of using NF1-GRD and AAV for gene replacement therapy in NF1-associated tumors
    Authors: RY Bai, D Esposito, AJ Tam, F McCormick, GJ Riggins, D Wade Clapp, V Staedtke
    Gene Ther., 2019-05-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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