HA Tag Antibody

Catalog # Availability Size / Price Qty
MAB060
MAB060-SP
Best Seller
Detection of N‑terminal HA-tag by Western Blot.
1 Image
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (8)

HA Tag Antibody Summary

Specificity
Specifically detects N-terminal HA-tagged proteins with a free N-terminus.
Source
Monoclonal Mouse IgG2B Clone # 543851
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HA Tag Peptide
Accession # ABB51961
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of N-terminal HA-tag antibody by Western Blot. View Larger

Detection of N‑terminal HA-tag by Western Blot. Western blot shows Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP, 5 ng/lane). PVDF membrane was probed with 1 µg/mL of Mouse Anti-HA Tag Peptide Antibody Monoclonal Antibody (Catalog # MAB060) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for N-terminal HA-tagged Recombinant Human APRIL/ TNFSF13 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HA Tag

Entrez Gene IDs
23308115 (Influenza A Virus H1N1); 3655151 (Influenza A Virus H3N2)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

Product Datasheets

You must select a language.

x

Citations for HA Tag Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target
    Authors: Dobaño-López, C;Valero, JG;Araujo-Ayala, F;Nadeu, F;Gava, F;Faria, C;Norlund, M;Morin, R;Bernes-Lasserre, P;Arenas, F;Grau, M;López, C;López-Oreja, I;Serrat, N;Martínez-Farran, A;Hernández, L;Playa-Albinyana, H;Giménez, R;Beà, S;Campo, E;Lagarde, JM;López-Guillermo, A;Magnano, L;Colomer, D;Bezombes, C;Pérez-Galán, P;
    Blood cancer journal
    Species: Human
    Sample Types: cell culture
  2. Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest
    Authors: Acharya BR, Fang JS, Jeffery ED et al.
    Life science alliance
  3. Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor Apoptosis
    Authors: Thomas J. Wubben, Sraboni Chaudhury, Brennan T. Watch, Jeanne A. Stuckey, Eric Weh, Roshini Fernando et al.
    Pharmaceuticals (Basel)
  4. Small molecule activation of metabolic enzyme pyruvate kinase muscle isozyme 2, PKM2, circumvents photoreceptor apoptosis
    Authors: Thomas J. Wubben, Mercy Pawar, Eric Weh, Andrew Smith, Peter Sajjakulnukit, Li Zhang et al.
    Scientific Reports
  5. Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
    Authors: J Dai, MA Luftig
    J. Immunol., 2018-01-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  6. Trichostatin A resistance is facilitated by HIF-1 alpha acetylation in HeLa human cervical cancer cells under normoxic conditions
    Authors: Jae-Wook Lee, Dong Hee Yang, Sojin Park, Hae-Kyoung Han, Jong-Wan Park, Bo Yeon Kim et al.
    Oncotarget
  7. FAS apoptotic inhibitory molecule 2 is a stress-induced intrinsic neuroprotective factor in the retina
    Authors: Mercy Pawar, Boris Busov, Aaruran Chandrasekhar, Jingyu Yao, David N Zacks, Cagri G Besirli
    Cell Death & Differentiation
  8. Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection
    Authors: AM Price, J Dai, Q Bazot, L Patel, PA Nikitin, R Djavadian, PS Winter, CA Salinas, AP Barry, KC Wood, EC Johannsen, A Letai, MJ Allday, MA Luftig
    Elife, 2017-04-20;6(0):.
    Species: Human
    Sample Types: Recombinant Protein
  9. Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
    Authors: Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer B, Kuppers R
    Proc Natl Acad Sci U S A, 2015-01-26;112(6):E546-55.
    Species: Human
    Sample Types: Protein
    Applications: Cross-linking
  10. Mitogen-induced B-cell proliferation activates Chk2-dependent G1/S cell cycle arrest.
    Authors: Nikitin, Pavel A, Price, Alexande, McFadden, Karyn, Yan, Christop, Luftig, Micah A
    PLoS ONE, 2014-01-30;9(1):e87299.
    Species: Human
    Sample Types: Protein
    Applications: Cross-linking
  11. Epstein-Barr Virus Induces Adhesion Receptor CD226 (DNAM-1) Expression during Primary B-Cell Transformation into Lymphoblastoid Cell Lines
    Authors: L Grossman, C Chang, J Dai, PA Nikitin, DD Jima, SS Dave, MA Luftig
    mSphere, 2017-11-29;2(6):.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for HA Tag Antibody

Average Rating: 4.8 (Based on 8 Reviews)

5 Star
87.5%
4 Star
0%
3 Star
12.5%
2 Star
0%
1 Star
0%

Have you used HA Tag Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


HA Tag Antibody
By Anonymous on 11/04/2021
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

HA Tag Antibody
By Lucia Sereni on 01/05/2021
Application: CellProlif Sample Tested: B cells Species: Mouse

HA Tag Antibody
By Anonymous on 10/22/2020
Application: Cell Culture Sample Tested: B cells Species: Mouse

This product has been used in combination with murine CD40L-HaTag protein to culture primary murine B cells.


HA Tag Antibody
By Kurt Shanebeck on 12/06/2017
Application: Func Sample Tested: Recombinant protein Species: Human

HA Tag Antibody
By Shub Poddar on 08/23/2017
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human

HA Tag Antibody
By Anonymous on 07/23/2017
Application: WB Sample Tested: 293-EBNA human EBV-expressing embryonic kidney cell line Species: Mouse

HA Tag Antibody
By Anonymous on 07/21/2017
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

HA Tag Antibody
By Anonymous on 07/20/2017
Application: WB Sample Tested: 293-EBNA human EBV-expressing embryonic kidney cell line Species: Mouse