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HA Tag Biotinylated Antibody

Catalog #: BAM0601
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
BAM0601
Detection of HA-tagged Proteins by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas

HA Tag Biotinylated Antibody Summary

Specificity
Detects N-terminal and C-terminal HA Tag proteins in Western blot.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1049F
Purification
Protein A or G purified from cell culture supernatant
Immunogen
HA Tag Peptide
Accession # ABB51961
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of HA-tagged Proteins antibody by Western Blot. View Larger

Detection of HA-tagged Proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-HA Tag Biotinylated Monoclonal Antibody (Catalog # BAM0601) followed by Streptavidin-HRP (Catalog # DY998). Specific bands were detected for HA-tagged proteins at approximately 70 and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HA Tag

Entrez Gene IDs
23308115 (Influenza A Virus H1N1); 3655151 (Influenza A Virus H3N2)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

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Citation for HA Tag Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Overexpression of prostaglandin E2 EP4 receptor improves cardiac function after myocardial infarction
    Authors: Timothy D. Bryson, Xiaosong Gu, Remonda M. Khalil, Safa Khan, Liping Zhu, Jiang Xu et al.
    Journal of Molecular and Cellular Cardiology

FAQs

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