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HA Tag Horseradish Peroxidase-conjugated Antibody

Catalog #: HAM0601
3 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
HAM0601
Detection of HA-tagged Proteins by Western Blot.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas

HA Tag Horseradish Peroxidase-conjugated Antibody Summary

Specificity
Detects N-terminal and C-terminal HA-tagged proteins in Western blot.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1049F
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HA Tag Peptide
Accession # ABB51961
Formulation
Supplied as a solution in PBS containing a preservative.
Label
HRP(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
1:1000-1:3000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of HA-tagged Proteins antibody by Western Blot. View Larger

Detection of HA-tagged Proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 1:1000-1:3000 dilution of Rabbit Anti-HA Tag Peptide HRP-conjugated Monoclonal Antibody (Catalog # HAM0601). Specific bands were detected for HA-tagged proteins at approximately 70 kDa and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: HA Tag

Entrez Gene IDs
23308115 (Influenza A Virus H1N1); 3655151 (Influenza A Virus H3N2)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

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Citations for HA Tag Horseradish Peroxidase-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag
    Authors: I Sahu, SM Mali, P Sulkshane, C Xu, A Rozenberg, R Morag, MP Sahoo, SK Singh, Z Ding, Y Wang, S Day, Y Cong, O Kleifeld, A Brik, MH Glickman
    Nature Communications, 2021-10-26;12(1):6173.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Structures of metabotropic GABAB receptor
    Authors: MM Papasergi-, MJ Robertson, AB Seven, O Panova, JM Mathiesen, G Skiniotis
    Nature, 2020-06-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA (detection)
  3. Salmonella Effector SteE Converts the Mammalian Serine/Threonine Kinase GSK3 into a Tyrosine Kinase to Direct Macrophage Polarization
    Authors: Ioanna Panagi, Elliott Jennings, Jingkun Zeng, Regina A. Günster, Cullum D. Stones, Hazel Mak et al.
    Cell Host & Microbe

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