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HB-2-30

Catalog #: 8831 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8831/100U
HB-2-30
3 Images
Description: Fluorescent TG2/LRP1 substrate for imaging endocytosis

Purity: ≥90%

Product Details
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Biological Activity Scientific Data Technical Data Additional Information Product Datasheets Calculators

Biological Activity

Key information: HB-2-30 is a far-red fluorescent transglutaminase-2 (TG2) probe for imaging endocytosis in vitro and in vivo. HB-2-30 undergoes highly efficient endocytosis in complex with TG2 and α2-macroglobulin via the LRP1 pathway.

Application: Confocal microscopy.

Properties and Photophysical Data: Suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining. Excitation and emission maxima (λ) are 649 and 665 nm, respectively.

Scientific Data

Confocal Microscopy Application of HB-2-30 in vitro View Larger

Application of HB-2-30 in vitro. Confocal microscopy images of bone marrow-derived dendritic cells from WT C57BL/6 mice (left) and TG2KO mice (right) incubated with 3μM HB-2-30. Cells were incubated for 90 minutes with the probe before being fixed, stained for nuclei (blue), and imaged. HB-2-30 signals is in red.

Confocal Microscopy Application of HB-2-30 in vivo. View Larger

Application of HB-2-30 in vivo. Ileum tissue sections from mice dosed with 10 mg/kg HB-2-30. WT C57BL/6 mice (left) and TG2KO mice (right) were dosed with HB-2-30 and sacrificed after 2 hours. Intestines were Swiss rolled, thin sectioned, stained for nuclei (blue), and imaged via confocal fluorescence microscopy. HB-2-30 signal is in red.

Optical Data for HB-2-30

λabs 649 nm
λem 665 nm
Application Confocal microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data

M.Wt:
1307.59
Formula:
C66H86N10O14S2
Sequence:
PXXPF (Modifications: Pro-1 = N-terminal Ac, X-2 = (S,E)-2-amino-7-(dimethylamino)-7-oxohept-5-enoic acid, X-3 = Dab(Sulfo-Cy5), Phe-5 = C-terminal amide)
Solubility:
Soluble to 13.08 mg/ml in DMSO
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
3031522-14-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from Stanford University

Product Datasheets

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