His Tag Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC050G
Detection of His Tag in HEK293 cell line transfected with His-tagged protein cells by Flow Cytometry.
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His Tag Alexa Fluor® 488-conjugated Antibody Summary

Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
His-tagged peptide
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.5 µg/106 cells
HEK293 cell line transfected with His-tagged protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry View Larger

Detection of His Tag in HEK293 cell line transfected with His-tagged protein cells by Flow Cytometry. HEK293 cell line transfected with His-tagged protein cells were stained with Mouse Anti-His Tag Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC050G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6-His Tag; 6X His Tag ; 6X His; 6x-His Tag; 6X-His; H; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; His6-Tag; polyhistidine tag; polyHistidine; Poly-histidine

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Reviews for His Tag Alexa Fluor® 488-conjugated Antibody

Average Rating: 5 (Based on 3 Reviews)

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His Tag Alexa Fluor® 488-conjugated Antibody
By Jenna Nguyen on 02/08/2019
Application: Affinity Purification Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse

His Tag Alexa Fluor® 488-conjugated Antibody
By Jenna Nguyen on 01/30/2019
Application: ELISA Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line Species: Mouse

His Tag Alexa Fluor® 488-conjugated Antibody
By Jenna Nguyen on 12/06/2018
Application: Cell Depl Sample Tested: 293T human embryonic kidney cell line Species: Human