His Tag Biotinylated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of His Tag by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-His Tag Biotinylated Monoclonal Antibody (Catalog # BAM050) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
Product Datasheets
Citations for His Tag Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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The FLRT3-UNC5B checkpoint pathway inhibits T cell-based cancer immunotherapies
Authors: Prajapati, K;Yan, C;Yang, Q;Arbitman, S;Fitzgerald, DP;Sharee, S;Shaik, J;Bosiacki, J;Myers, K;Paucarmayta, A;Johnson, DM;O'Neill, T;Kundu, S;Cusumano, Z;Langermann, S;Langenau, DM;Patel, S;Flies, DB;
Science advances
Species: N/A
Sample Types: Recombinant Protein
Applications: ELISA Detection -
Working strokes produced by curling protofilaments at disassembling microtubule tips can be biochemically tuned and vary with species
Authors: LE Murray, H Kim, LM Rice, CL Asbury
Elife, 2022-12-29;11(0):.
Species: Bovine, Yeast
Sample Types:
Applications: Bioassay -
Candida albicans elicits protective allergic responses via platelet mediated T helper 2 and T helper 17 cell polarization
Authors: Yifan Wu, Zhimin Zeng, Yubiao Guo, Lizhen Song, Jill E. Weatherhead, Xinyan Huang et al.
Immunity
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Affinity-matured HLA class II dimers for robust staining of antigen-specific CD4+ T cells
Authors: K Sugata, Y Matsunaga, Y Yamashita, M Nakatsugaw, T Guo, L Halabelian, Y Ohashi, K Saso, MA Rahman, M Anczurowsk, CH Wang, K Murata, H Saijo, Y Kagoya, D Ly, BD Burt, MO Butler, TW Mak, N Hirano
Nature Biotechnology, 2021-03-01;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
IgG-cleaving endopeptidase enables in vivo gene therapy in the presence of anti-AAV neutralizing antibodies
Authors: C Leborgne, E Barbon, JM Alexander, H Hanby, S Delignat, DM Cohen, F Collaud, S Muraleetha, D Lupo, J Silverberg, K Huang, L van Witten, B Marolleau, A Miranda, A Fabiano, V Daventure, H Beck, XM Anguela, G Ronzitti, SM Armour, S Lacroix-De, F Mingozzi
Nat. Med., 2020-06-01;0(0):.
Species: Cynomolgus Monkey
Sample Types: Plasma
Applications: ELISA Detection -
Discovery and characterization of single-domain antibodies for polymeric Ig receptor-mediated mucosal delivery of biologics
Authors: Bharathikumar Vellalore Maruthachalam, Adam Zwolak, Xiefan Lin-Schmidt, Edward Keough, Ninkka Tamot, Sathya Venkataramani et al.
mAbs
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Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
Cell Rep, 2019-06-11;27(11):3117-3123.e5.
Species: Human
Sample Types: Cell Culture Supernates, Protein
Applications: ELISA Development, Epitope Mapping -
Antibodies Targeting Closely Adjacent or Minimally Overlapping Epitopes Can Displace One Another
Authors: YN Abdiche, AY Yeung, I Ni, D Stone, A Miles, W Morishige, A Rossi, P Strop
PLoS ONE, 2017-01-06;12(1):e0169535.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development -
Assessing kinetic and epitopic diversity across orthogonal monoclonal antibody generation platforms
Authors: Yasmina Noubia Abdiche, Rian Harriman, Xiaodi Deng, Yik Andy Yeung, Adam Miles, Winse Morishige et al.
mAbs
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High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity.
Authors: Abdiche, Yasmina, Miles, Adam, Eckman, Josh, Foletti, Davide, Van Blarcom, Thomas J, Yeung, Yik Andy, Pons, Jaume, Rajpal, Arvind
PLoS ONE, 2014-03-20;9(3):e92451.
Applications: Bioassay -
Designed ankyrin repeat proteins: a new approach to mimic complex antigens for diagnostic purposes?
Authors: Hausammann, Stefanie, Vogel, Monique, Kremer Hovinga, Johanna, Lacroix-Desmazes, Sebastie, Stadler, Beda M, Horn, Michael
PLoS ONE, 2013-04-23;8(4):e60688.
Species: Human
Sample Types:
Applications: Binding Assay -
Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
Int. J. Cancer, 2006-07-15;119(2):349-58.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
FAQs
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Can the His Tag Antibody (MAB050) recognize all His-tagged proteins?
MAB050 has been validated specifically for the detection of His-tags localized to the amino- or carboxyl-terminus of a peptide. It is also expected to detect any series of 6 or more histidine residues as long as this epitope is conformationally accessible. For applications utilizing linearized proteins, it is likely that MAB050 will have access to any consecutive histidine regions. If used with protein in its native conformation, there is the potential that the protein will be folded such that the antibody is not able to bind its target.
Reviews for His Tag Biotinylated Antibody
Average Rating: 4.2 (Based on 6 Reviews)
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Antibody was used to probe cell lysates from E. coli to identify protein of interest which carries a C-terminal his-tag. The yield of our protein is very low in the soluble fraction, so we used this mAb to compare the soluble fraction to the inclusion body prep to see if there was a signal in the inclusion body prep indicating that our protein may not be soluble.
This antibody was used to capture his tagged proteins in various biosensor assays. This antibody works fine for general purposes. In some platforms (i.e., Octet), it is not as robust as a capture reagent, but it is still acceptable.
