His Tag PE-conjugated Antibody

Catalog #: IC0501P Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
IC0501P-100

Detection of protein binding with Mouse anti-His Tag PE in HEK293 cells by Flow Cytometry

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His Tag PE-conjugated Antibody Summary

Specificity

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags located at the amino- or carboxyl- terminus

Source

Monoclonal Mouse IgG₁ Clone # AD1.1.10R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

His-tagged peptide

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

HEK293 cells transfected with Human ACE-2 and eGFP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of protein binding with Mouse anti-His Tag PE in HEK293 cells by Flow Cytometry In a functional flow cytometry test, recombinant SARS-CoV2 S1 RBD His-tagged protein (50 ng/mL, 10500-CV) binds to HEK293 cells transfected with Human ACE-2 and eGFP. (A) Protein binding was detected with Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) or Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) alone. View our protocol for View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

6 His epitope tag; 6-His Tag; 6X His Tag ; 6X His; 6x-His Tag; 6X-His; H; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; His6-Tag; polyhistidine tag; polyHistidine; Poly-histidine