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HT F Homogeneous PARP Inhibition Assay Kit

Catalog #: 4690-096-K
1 Citation Datasheet / COA / SDS
For the measurment of PARP-1 activity in cell extracts

Discontinued Product

4690-096-K has been discontinued.
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Product Details
Citations (1)
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Summary Product Datasheets

HT F Homogeneous PARP Inhibition Assay Kit Summary

Enables the high-throughput in vitro screening of candidate PARP-1 inhibitors and determination of relative IC50 values.
 

Key Benefits

• Highly sensitive fluorescent assay
• Detect as little as 10% inhibition of PARP Activity
• Inhibitors identified by increase in Fluorescent Signal
• No wash steps
• 96 test size
• Rapid – Assay takes ~ 1.5 hr

Other Resources

4690-096-k_calculation_worksheetv2

Product Specifications

For the screening of candidate PARP inhibitors and determination of relative IC50 values.

Kit Contents

• 10X Cycling Enzymes
• 2 µM NAD
• 20 mM NAD
• PARP 1 Enzyme
• Activated DNA
• 10X Resazurin
• 10X Buffer
• Stop Solution
• 96-well Black Microplate

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

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COA

Citation for HT F Homogeneous PARP Inhibition Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. RAD52 Adjusts Repair of Single-Strand Breaks via Reducing DNA-Damage-Promoted XRCC1/LIG3&alpha Co-localization
    Authors: J Wang, YT Oh, Z Li, J Dou, S Tang, X Wang, H Wang, S Takeda, Y Wang
    Cell Reports, 2021-01-12;34(2):108625.  2021-01-12

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