HT gamma -H2AX Pharmacodynamic Assay
HT gamma -H2AX Pharmacodynamic Assay Summary
Enables the quatification of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies.Key Benefits
• Chemiluminescent, non-radioactive ELISA
• Pre-coated 96 well capture antibody plate
• Dynamic range from 10 pM to 800pM
• Sensitivity with 5 pM of gamma-H2AX
Product Specifications
Quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies.
Other Resources
4418-096-k_calculation_worksheet
Kit Contents
• Gamma-H2AX Standard, 1 µM
• Jurkat Cell Lysate Control
• Assay Buffer
• Cell Lysis Reagent
• 25X Wash Buffer
• 96-Stripwell pre-coated plate with 3 film sealers
• PARP PeroxyGlow A
• PARP PeroxyGlow B
• H2AX IgM Detecting Antibody
• Goat anti-Mouse IgM HRP Conjugate
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for HT gamma -H2AX Pharmacodynamic Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Ordinary Gasoline Emissions Induce a Toxic Response in Bronchial Cells Grown at Air-Liquid Interface
Authors: T Cervena, M Vojtisek-L, K Vrbova, A Ambroz, Z Novakova, F Elzeinova, M Sima, V Beranek, M Pechout, D Macoun, J Klema, A Rossnerova, M Ciganek, J Topinka, P Rossner
International Journal of Molecular Sciences, 2020-12-23;22(1):. 2020-12-23
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S-Adenosylmethionine Treatment of Colorectal Cancer Cell Lines Alters DNA Methylation, DNA Repair and Tumor Progression-Related Gene Expression
Authors: S Zsigrai, A Kalmár, ZB Nagy, BK Barták, G Valcz, KA Szigeti, O Galamb, T Dankó, A Sebestyén, G Barna, V Szabó, O Pipek, A Medgyes-Ho, I Csabai, Z Tulassay, P Igaz, I Takács, B Molnár
Cells, 2020-08-09;9(8):. 2020-08-09
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The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAirTM) and in Human Bronchial Epithelial Cells (BEAS-2B)
Authors: P Rossner, T Cervena, M Vojtisek-L, K Vrbova, A Ambroz, Z Novakova, F Elzeinova, H Margaryan, V Beranek, M Pechout, D Macoun, J Klema, A Rossnerova, M Ciganek, J Topinka
Int J Mol Sci, 2019-11-14;20(22):. 2019-11-14
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Improved Therapeutic Window in BRCA-mutant Tumors with Antibody-linked Pyrrolobenzodiazepine Dimers with and without PARP Inhibition
Authors: H Zhong, C Chen, R Tammali, S Breen, J Zhang, C Fazenbaker, M Kennedy, J Conway, BW Higgs, N Holoweckyj, R Raja, J Harper, AJ Pierce, R Herbst, DA Tice
Mol. Cancer Ther., 2018-10-23;18(1):89-99. 2018-10-23
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