Human 17 beta-HSD14/HSD17B14 Antibody

Name: Human 17 beta-HSD14/HSD17B14 Antibody
Brand: R&D Systems
SKU: MAB7515
Rating: 5 (2 reviews)

Catalog #: MAB7515 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7515
MAB7515-SP

1 Image

All 17 beta-HSD14/HSD17B14 Antibodies

Product Details

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 17 beta-HSD14/HSD17B14 Antibody Summary

Species Reactivity

Human

Specificity

Detects human 17 beta ‑HSD14/HSD17B14 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 736043

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human 17 beta ‑HSD14/HSD17B14
Ala2-Ser270
Accession # Q9BPX1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

17 beta ‑HSD14/HSD17B14 in Human Brain. 17 beta -HSD14/HSD17B14 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human 17 beta -HSD14/HSD17B14 Monoclonal Antibody (Catalog # MAB7515) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 17 beta-HSD14/HSD17B14

HSD-17b14 (hydroxysteroid (17-beta) dehydrogenase type14), also called 17bHSD14 or DHRS10, is a cytoplasmic 17beta-hydroxysteroid dehydrogenase that is a member of the SDR (short-chain dehydrogenase/reductase) family. It is a 270 amino acid, approximately 30 kDa protein with mRNA highly expressed in brain, placenta, liver and kidney. It converts estradiol to estrone, thus inactivating it, and is thought to regulate activity of estrogens and possibly dihydroepiandrosterone (DHEA) in the placenta and central nervous system. Human HSD-17b14 shares 80.5% amino acid sequence identity with mouse and rat HSD-17b14.

Long Name

17 beta Hydroxysteroid Dehydrogenase 14

Entrez Gene IDs

51171 (Human); 66065 (Mouse); 691018 (Rat)

Alternate Names

17 betaHSD14; 17 beta-HSD14; dehydrogenase/reductase (SDR family) member 10; Dehydrogenase/reductase SDR family member 10; DHRS10; DHRS1017-beta-hydroxysteroid dehydrogenase 14; EC 1.1.1.-; HSD17B14; hydroxysteroid (17-beta) dehydrogenase 14,17-beta-hydroxysteroid dehydrogenase DHRS10; retinal short-chain dehydrogenase/reductase 3; Retinal short-chain dehydrogenase/reductase retSDR3; retSDR3; RETSDR3,17-beta-HSD 14; SDR3; SDR47C1; short chain dehydrogenase/reductase family 47C, member 1