Human 20S Immunoproteasome Protein, CF
Human 20S Immunoproteasome Protein, CF Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-370
Formulation | Supplied as a solution in HEPES, NaCl and DTT. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: 20S Immunoproteasome
The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi-complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged into four stacked rings (1,2). However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, 2, and 5, in the two interior rings of the 20S Proteasome are replaced by three IFN-gamma-inducible catalytic subunits: beta 1i/LMP2, beta 2i/LMP7, and beta 5i/MECL-1 (3). The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha/beta, or the PA28 gamma regulatory complexes (3,4). 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation (3,5,6). Selective inhibition of 20S Immunoproteasome proteolytic activity using small molecule inhibitors is being examined for therapeutic intervention in cancer and inflammatory diseases (7).
This protein has been purified from human peripheral blood mononuclear cells, which have been screened and are negative for hepatitis B surface antigen, antibodies to hepatitis C virus, HIV type 1 antigens, and antibodies to HIV type 1 and 2.
.- Kim, H.M. et al. (2011) Biochim. Biophys. Acta 1809:67.
- Xie, Y. (2010) J. Mol. Cell Biol. 2:308.
- Kloetzel, P.M. (2001) Nat. Rev. Mol. Cell Biol. 2:179.
- Stadtmueller, B.M. & C.P. Hill (2011) Mol. Cell 41:8.
- Cascio, P. et al. (2001) EMBO J. 20:2357.
- Ferrington, D.A. & D.S. Gregerson (2012) Prog. Mol. Biol. Transl. Sci. 109:75.
- Lee, W. & K.B. Kim (2011) Curr. Top. Med. Chem. 11:2923.
Citations for Human 20S Immunoproteasome Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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SARS-CoV-2 mutations affect antigen processing by the proteasome to alter CD8+ T cell responses
Authors: Wellington, D;Yin, Z;Yu, Z;Heilig, R;Davis, S;Fischer, R;Felce, SL;Antoun, E;Hublitz, P;Beveridge, R;Dong, D;Liu, G;Yao, X;Peng, Y;Kessler, BM;Dong, T;
Heliyon
Species: N/A
Sample Types: Peptide
Applications: Bioassay -
Discovery of Immunoproteasome Inhibitors Using Large-Scale Covalent Virtual Screening
Authors: A Scarpino, D Bajusz, M Proj, M Gobec, I Sosi?, S Gobec, GG Ferenczy, GM Keser?
Molecules, 2019-07-16;24(14):.
Species: Human
Sample Types: Small Molecule
Applications: Bioassay -
Development of a novel immunoproteasome digestion assay for synthetic long peptide vaccine design
Authors: H Wada, A Shimizu, T Osada, Y Tanaka, S Fukaya, E Sasaki
PLoS ONE, 2018-07-03;13(7):e0199249.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Piperlongumine and some of its analogs inhibit selectively the human immunoproteasome over the constitutive proteasome
Authors: E Bosc, J Nastri, V Lefort, M Valli, F Contiguiba, R Pioli, M Furlan, VDS Bolzani, C El Amri, M Reboud-Rav
Biochem. Biophys. Res. Commun., 2018-02-02;496(3):961-966.
Species: Human
Sample Types: Small Molecule
Applications: Bioassay -
Immunoproteasome functions explained by divergence in cleavage specificity and regulation
Authors: MB Winter, F La Greca, S Arastu-Kap, F Caiazza, P Cimermanci, TJ Buchholz, JL Anderl, M Ravalin, MF Bohn, A Sali, AJ O'Donoghue, CS Craik
Elife, 2017-11-28;6(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes
Authors: RLA Santos, L Bai, PK Singh, N Murakami, H Fan, W Zhan, Y Zhu, X Jiang, K Zhang, JP Assker, CF Nathan, H Li, J Azzi, G Lin
Nat Commun, 2017-11-22;8(1):1692.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens
Authors: AY Chang, T Dao, RS Gejman, CA Jarvis, A Scott, L Dubrovsky, MD Mathias, T Korontsvit, V Zakhaleva, M Curcio, RC Hendrickso, C Liu, DA Scheinberg
J. Clin. Invest., 2017-06-19;127(7):2705-2718.
Applications: Enzyme Assay -
Clinical activity of carfilzomib correlates with inhibition of multiple proteasome subunits: application of a novel pharmacodynamic assay
Authors: SJ Lee, K Levitsky, F Parlati, MK Bennett, S Arastu-Kap, L Kellerman, TF Woo, AF Wong, KP Papadopoul, R Niesvizky, AZ Badros, R Vij, S Jagannath, D Siegel, M Wang, GJ Ahmann, CJ Kirk
Br J Haematol, 2016-04-12;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Enzymatic discovery of a HER-2/neu epitope that generates cross-reactive T cells.
Authors: Henle A, Erskine C, Benson L, Clynes R, Knutson K
J Immunol, 2012-11-23;190(1):479-88.
Applications: Bioassay
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Ordinate: fluorescent count, Em 345/Ex 445
Em band path: 9 nm
Ex band path: 20 nm
Horizontal: time [sec]
Temp: 37 °C
1. i20S(15 nM)/PA28a(150 nM)/S-300(0.01 uM)
2. i20S(15 nM)/PA28a(150 nM)/S-300(0.1 uM)
3. i20S(15 nM)/PA28a(150 nM)/S-300(1 uM)
4. 20S(15 nM)/PA28a(150 nM)/S-300(0.01 uM)
5. 20S(15 nM)/PA28a(150 nM)/S-300(0.1 uM)
6. 20S(15 nM)/PA28a(150 nM)/S-300(1 uM)
The hydrolysis activity of proteasome and immunoproteasome is considered to be lower than that of trypsin. About 15 nM is required in the co-presence of PA28 activator. At that time, the substrate concentration should be about 1 uM for the observation of the saturation level.