Human 4-1BB/TNFRSF9/CD137 PE-conjugated Antibody

Catalog #: FAB838P Datasheet / COA / SDS

Discontinued Product

FAB838P has been discontinued.
View all 4-1BB/TNFRSF9/CD137 products.
Detection of 4‑1BB/TNFRSF9/CD137 in Human PBMCs by Flow Cytometry.
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Human 4-1BB/TNFRSF9/CD137 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB, recombinant human (rh) CD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLTR beta, rmLTR beta, rhNGFR, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII is observed.
Source
Monoclonal Mouse IgG2B Clone # 145501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human 4‑1BB/TNFRSF9/CD137
Leu24-Gln186
Accession # Q07011
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of 4-1BB/TNFRSF9/CD137 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of 4‑1BB/TNFRSF9/CD137 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA for 5 hours were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 4-1BB/TNFRSF9/CD137 PE-conjugated Monoclonal Antibody (Catalog # FAB838P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: 4-1BB/TNFRSF9/CD137

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 KD glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid sequence identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

Entrez Gene IDs
3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
Alternate Names
4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9

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