Human 5-HT2A Antibody

Catalog #: MAB112501 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB112501-100
MAB112501-SP

Detection of 5-HT2A in NS0 mouse myeloma cell line transfected (positive).

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 5-HT2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human HTR2A in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1055220

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide corresponding to the amino acids 1-75 in the N-term extracellular domain of the Serotonin Receptor 5-HT2A (HTR2A).
Accession # P28223

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.5 µg/10⁶ cells

HEK293 cells transfected with Human HT2A and eGFP vs irrelevant

Immunocytochemistry

8-25 µg/mL

Immersion fixed NS0 mouse myeloma cell line transfected (positive) and NS0 mouse myeloma cell line wild type (negative) cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of 5-HT2A in NS0 mouse myeloma cell line transfected (positive). 5-HT2A was detected in immersion fixed NS0 mouse myeloma cell line transfected (positive) cells and absent in NS0 mouse myeloma cell line wild type (negative) cells using Mouse Anti-Human 5-HT2A Monoclonal Antibody (Catalog # MAB112501) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry

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Detection of 5-HT2A in HEK293 cells transfected with Human HT2A and eGFP vs irrelevant cells by Flow Cytometry. HEK293 cells transfected with Human HT2A and eGFP vs irrelevant were stained with eGFP and Mouse Anti-Human 5-HT2A Monoclonal Antibody (A, B) (Catalog # MAB112501) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT2A

Human serotonin receptor HTR2A, also known as 5-HT2A or HTR2, is a G-protein coupled receptor (GPCR) for 5-hydroxytryptamine (serotonin). The 5-HT2A receptor is mainly a cell surface receptor with several intracellular locations. Human -5-HT2A is the main excitatory receptor subtype among the GPCRs for serotonin but an inhibitory effect on the visual cortex and the orbitofrontal cortex has also been described. This receptor was first noted for its importance as a target of serotonergic psychedelic drugs such as LSD and psilocybin mushrooms.

Long Name

5-Hydroxytryptamine Receptor 2A

Entrez Gene IDs

3356 (Human); 15558 (Mouse); 29595 (Rat)

Alternate Names

5-HT2 receptor; 5-HT-2; 5HT2A; 5-HT2A; 5-HT-2A; 5-hydroxytryptamine (serotonin) receptor 2A; 5-hydroxytryptamine receptor 2A; HTR2,5-HT2A; HTR2A; serotonin 5-HT-2A receptor; Serotonin receptor 2A