Human 5-HT2B APC-conjugated Antibody

Catalog #: FAB11297A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB11297A-100

Detection of 5-HT2B in HEK293 cell transfected with 5-HT2B and eGFP vs irrelevant cells by Flow Cytometry.

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Human 5-HT2B APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human 5-HTR2B in direct ELISA.

Source

Monoclonal Rabbit IgG Clone # 2880B

Purification

Protein A or G purified from ascites

Immunogen

Synthetic peptide containing human 5- HTR2B
Accession # P41595

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

HEK293 cell transfected with 5-HT2B and eGFP vs irrelevant

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of 5-HT2B in HEK293 cell transfected with 5-HT2B and eGFP vs irrelevant cells by Flow Cytometry. HEK293 cell transfected with 5-HT2B and eGFP vs irrelevant was stained with eGFP and Rabbit Anti-Human 5-HT2B APC‑conjugated Monoclonal Antibody (Catalog # FAB11297A) in 5-HT2B transfectants (A) or (B) irrelevant transfectants. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: 5-HT2B

HTR2B is a G-protein coupled receptor for serotonin. The 5HT2 receptors mediate many of the central and peripheral physiologic functions of serotonin. 5HT2B plays a role in the regulation of dopamine and serotonin release, serotonin uptake and in the regulation of extracellular dopamine and serotonin levels. It also plays a role in pulmonary vasoconstriction and regulating cardiac structure and functions. 5HT2B receptors were shown to be expressed in human failing heart and have been strongly implicated in drug induced valvular heart disease.

Long Name

5-Hydroxytryptamine Receptor 2B

Entrez Gene IDs

3357 (Human); 15559 (Mouse); 29581 (Rat)

Alternate Names

5-HT(2B); 5HT2B; 5-HT2B; 5-HT-2B; 5-HT2B5-HT 2B receptor; 5-hydroxytryptamine (serotonin) receptor 2B; 5-hydroxytryptamine 2B receptor; 5-hydroxytryptamine receptor 2B; HTR2B; Serotonin receptor 2B