Human 5-HT4 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection 5-HT4 in HEK293 Human Cell Line Transfected with Human 5-HT4 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP was stained with (A) Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) or (B) Mouse IgG2B isotype control antibody (IC0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.
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5-HT4 in Human Brain. 5-HT4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 5-HT4
5-HT4 (5-hydroxytryptamine receptor 4), also known as HTR4 or 5HT4R, is an approximately 45 kDa multipass serotonin receptor that is expressed in the brain, gastrointestinal tract, bladder, vascular endothelial cells, atrium, kidney, and adrenal gland. It is involved in the regulation of learning, memory, anxiety, smooth muscle contraction, and adrenal corticosteroid secretion. Alternate splicing of human 5-HT4 generates multiple isoforms with either a 14 aa insertion in the second extracellular loop or a deletion or various substitutions in the C-terminal cytoplasmic region. Different isoforms show tissue specific expression patterns or increased activity in the absence of ligand. Human 5-HT4 shares 94% and 88% aa sequence identity with mouse and rat 5-HT4, respectively.
Product Datasheets
Citation for Human 5-HT4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory
Authors: Anna G. Orr, Edward C. Hsiao, Max M. Wang, Kaitlyn Ho, Daniel H. Kim, Xin Wang et al.
Nature Neuroscience
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