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Human 5'-Nucleotidase/CD73 APC-conjugated Antibody

Catalog #: FAB5795A
4 Citations Datasheet / COA / SDS
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FAB5795A
Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 5'-Nucleotidase/CD73 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.
Source
Monoclonal Mouse IgG2B Clone # 606112
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human 5'‑Nucleotidase/CD73
Trp27-Ile511
Accession # P21589
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µL/106 cells
Human peripheral blood mononuclear cells (PBMCs)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of 5'-Nucleotidase/CD73 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 APC-conjugated Monoclonal Antibody (Catalog # FAB5795A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of 5'‑Nucleotidase/CD73 in PBMC lymphocytes by Flow Cytometry. PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human 5'‑Nucleotidase/CD73 APC‑conjugated Monoclonal Antibody (Catalog # FAB5795A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: 5'-Nucleotidase/CD73

5'-Nucleotidase (also [ecto]-5'-nucleotidase/5'-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5'-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3+ Treg lymphocytes, FDCs and B cells. 5'-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5'-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5'‑Nucleotidase shares 88% aa identity with both mouse and rat 5'-Nucleotidase.

Entrez Gene IDs
4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)
Alternate Names
5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase

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Citations for Human 5'-Nucleotidase/CD73 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients
    Authors: Francisco Raúl RA贚 BORZONE, María Belén BEL蒒 GIORELLO, Leandro Marcelo MARCELO MARTINEZ, María Cecilia CECILIA SANMARTIN, LEONARDO FELDMAN, FEDERICO DIMASE et al.
    Oncology Research
  2. Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
    Authors: Milián, L;Molina, P;Oliver-Ferrándiz, M;Fernández-Sellers, C;Monzó, A;Sánchez-Sánchez, R;Braza-Boils, A;Mata, M;Zorio, E;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation
    Authors: J Liu, X Wei, J Hu, X Tan, X Kang, L Gao, N Li, X Shi, M Yuan, W Hu, M Liu
    BMC Oral Health, 2021-09-24;21(1):470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The neovascularization effect of dedifferentiated fat cells
    Authors: H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto
    Sci Rep, 2020-06-08;10(1):9211.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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