Human 5'-Nucleotidase/CD73 PE-conjugated Antibody Summary
Trp27-Ile511
Accession # P21589
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 PE-conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
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Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 PE-conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (IC0041P). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: 5'-Nucleotidase/CD73
5'-Nucleotidase (also [ecto]-5'-nucleotidase/5'-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5'-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3+ Treg lymphocytes, FDCs and B cells. 5'-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5'-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5'‑Nucleotidase shares 88% aa identity with both mouse and rat 5'-Nucleotidase.
Product Datasheets
Citations for Human 5'-Nucleotidase/CD73 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Fibroblast growth factor-6 enhances CDK2 and MATK expression in microvesicles derived from human stem cells extracted from exfoliated deciduous teeth
Authors: Ahmed Othman, Rabab Mubarak, Dina Sabry
F1000Research
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Infrared diode laser enhances human periodontal ligament stem cells behaviour on titanium dental implants
Authors: Abo El-Dahab, MM;El Deen, GN;Aly, RM;Gheith, M;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Inhibition of the Inducible Nitric Oxide Synthase Enhances the DPSC Mineralization under LPS-Induced Inflammation
Authors: A Cataldi, R Amoroso, V di Giacomo, S Zara, C Maccallini, M Gallorini
International Journal of Molecular Sciences, 2022-11-23;23(23):.
Species: Human
Sample Types: Whole Cells
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Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
Authors: A Borojevi?, A Jaukovi?, T Kukolj, S Mojsilovi?, H Obradovi?, D Trivanovi?, M Živanovi?, Ž Ze?evi?, M Simi?, B Gobelji?, D Vuji?, D Bugarski
Biomolecules, 2022-02-18;12(2):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Liposomes embedded with differentiating factors as a new strategy for enhancing DPSC osteogenic commitment
Authors: M Gallorini, R Di Carlo, S Pilato, A Ricci, H Schweikl, A Cataldi, A Fontana, S Zara
European cells & materials, 2021-01-27;41(0):108-120.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells
Authors: SJ Li, Y Luo, LM Zhang, W Yang, GG Zhang
Mol Med Rep, 2017-01-19;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors
Authors: Christopher J Hewitt
Biotechnol J, 2016-02-29;11(4):473-86.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
Stem Cells Dev, 2013-12-04;23(5):541-54.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Relevance of Cellular Redox Homeostasis for Vital Functions of Human Dental Pulp Cells
Authors: Marialucia Gallorini, Matthias Widbiller, Carola Bolay, Simone Carradori, Wolfgang Buchalla, Amelia Cataldi et al.
Antioxidants (Basel)
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