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Human 53BP1 Antibody

Catalog #: AF1877
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1877
AF1877-SP
Detection of Human 53BP1 by Western Blot.
3 Images
Product Details
Citations (4)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 53BP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human 53BP1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human 53BP1
Ala1614-His1972
Accession # Q12888
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Knockout Validated
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human 53BP1 antibody by Western Blot. View Larger

Detection of Human 53BP1 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line and ZR-75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human 53BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1877) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 53BP1 at approximately 350 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry 53BP1 antibody in Rat Kidney by Immunohistochemistry (IHC-Fr). View Larger

53BP1 in Rat Kidney. 53BP1 was detected in perfusion fixed frozen sections of rat kidney using Goat Anti-Human 53BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1877) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Knockout Validated Western Blot Shows Human 53BP1 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human 53BP1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 53BP1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 53BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1877) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 53BP1 at approximately 350 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 53BP1

The p53-binding protein 1 (53BP1) localizes to sites of damaged DNA and is phosphorylated by the ATM checkpoint kinase. 53BP1 is required for phosphorylation of other downstream ATM substrates, such as p53 and SMC-1, and therefore aids in nucleating a DNA damage response protein complex. Over aa 1614-1972, human 53BP1 shares 97% aa sequence identity with rat and mouse 53BP1. 

Long Name
p53 Binding Protein 1
Entrez Gene IDs
7158 (Human); 27223 (Mouse); 296099 (Rat)
Alternate Names
53BP1; p202; p53-binding protein 1; p53bp1; TDRD30; TP53-Binding Protein 1; TP53BP1; tumor protein 53-binding protein, 1; tumor protein p53 binding protein 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1

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Citations for Human 53BP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance
    Authors: Fei Li, Yizhe Wang, Inah Hwang, Ja-Young Jang, Libo Xu, Zhong Deng et al.
    Nature Communications
  2. Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance
    Authors: F Li, Y Wang, I Hwang, JY Jang, L Xu, Z Deng, EY Yu, Y Cai, C Wu, Z Han, YH Huang, X Huang, L Zhang, J Yao, NF Lue, PM Lieberman, H Ying, J Paik, H Zheng
    bioRxiv : the preprint server for biology, 2023-02-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: FISH
  3. Low-Dose Pesticides Alter Primary Human Bone Marrow Mesenchymal Stem/Stromal Cells through ALDH2 Inhibition
    Authors: A Foucault, N Ravalet, J Besombes, F Picou, N Gallay, L Babin, J Bourgeais, S Hamard, J Domenech, P Loyer, N Vallet, J Lejeune, E Gyan, MC Béné, F Vallette, C Olivier, O Hérault
    Cancers, 2021-11-14;13(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. ?-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes
    Sci Rep, 2016-12-06;6(0):38295.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC

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