Human A-Raf Antibody

Catalog #: MAB6607 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6607
MAB6607-SP

Detection of Human A‑Raf by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human A-Raf Antibody Summary

Species Reactivity

Human

Specificity

Detects human A-Raf in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B-Raf, rhRaf-1 (aa 1-648), rhRaf-1 (aa 189-353), or rhKSR1 is observed. In Western blots, no cross-reactivity with recombinant human (rh) B-Raf or rhKSR1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 684128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human A-Raf
Asn150-Lys314
Accession # P10398

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human A‑Raf by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human A-Raf Monoclonal Antibody (Catalog # MAB6607) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for A-Raf at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A-Raf

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: A-Raf, B-Raf, and Raf-1, also known as C-Raf. Human A-Raf is a 70 kDa protein that shares three regions with B-Raf and Raf-1: aa 14-153 which contains a Ras-binding domain, a Cys-rich domain, and a lipid-binding domain; aa 209-223; and aa 308-573 which contains the Ser/Thr protein kinase domain and a second lipid-binding domain. A-Raf is activated by phosphorylation at Ser 257, 262, and 264. It then regulates multiple processes including endocytic trafficking, glycolysis, cell cycle progression, and apoptosis. Within aa 150-314, human A-Raf shares 87% aa sequence identity with mouse and rat A-Raf.

Long Name

v-Raf Murine Sarcoma 3611 Viral Oncogene Homolog

Entrez Gene IDs

369 (Human)

Alternate Names

ARaf; A-Raf; ARAF1A-RAF; EC 2.7.11; EC 2.7.11.1; Oncogene ARAF1; PKS; PKS2; PKS2A-Raf proto-oncogene serine/threonine-protein kinase; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; RAFA1; Ras-binding protein DA-Raf; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene homolog