Human Activin RIB/ALK-4 Antibody

Catalog #: MAB222 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB222
MAB222-SP

Detection of Human Activin RIB/ALK‑4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Activin RIB/ALK-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Activin RIB/ALK-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 12% cross reactivity with recombinant human (rh) Activin RIA is observed. No cross reactivity is observed with rhActivin RIIA, or rhActivin RIIB.

Source

Monoclonal Mouse IgG₁ Clone # 126822

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Activin RIB/ALK-4
Ser24-Glu126
Accession # AAA60556

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HEK293 human embryonic kidney cell line transfected with human Activin RIB/ALK-4

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.2-1 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Cripto 1 (Catalog # 145-CR) to immobilized Recombinant Human Activin RIB/ALK-4 Fc Chimera (Catalog # 808-AR) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Activin RIB/ALK‑4 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line mock transfected or transfected with human Activin RIB/ALK-4. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Activin RIB/ALK‑4 Monoclonal Antibody (Catalog # MAB222) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Activin RIB/ALK‑4 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIB/ALK-4

Activin RIB, also known as ALK4, is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with and phosphorylates the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature human Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, human Activin RIB shares 93% and 95% aa sequence identity with mouse and rat Activin RIB, respectively. It shares 25-35% aa sequence identity with other human type I receptors Activin RIA, Activin RIC, BMPR-IA, BMPR-IB, and TGF-beta R1. Alternately spliced isoforms of Activin RIB have deletions in the cytoplasmic domain and function as dominant negative inhibitors of activin signaling (5, 6). Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (7-9). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (10). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation and apoptosis, and tumorigenesis (1, 11, 12).

References

Chen, Y- G. et al. (2006) Exp. Biol. Med. 231:534.
Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
Tsuchida, K. et al. (1995) Endocrinology 136:5493.
ten Dijke, P. et al. (1993) Oncogene 8:2879.
Xu, J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:7957.
Zhou, Y. et al. (2000) Mol. Endocrinol. 14:2066.
Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
Lewis, K.A. et al. (2000) Nature 404:411.
Gu, Z. et al. (1998) Genes Dev. 12:844.
Chen, Y. et al. (2004) Dev. Biol. 268:280.

Long Name

Activin Receptor IB

Entrez Gene IDs

91 (Human); 11479 (Mouse)

Alternate Names

activin A receptor, type IB; Activin receptor type IB; Activin receptor-like kinase 4; Activin RIB; ActivinRIB; ACTRIB; ACVR1B; ACVRLK4ACTR-IB; ALK4; ALK-4; ALK4activin A receptor, type II-like kinase 4; EC 2.7.11; EC 2.7.11.30; Serine/threonine-protein kinase receptor R2; SKR2activin receptor type-1B